LOCUS       sequence-342379-        4578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC25A13_STOP
FEATURES             Location/Qualifiers
     source          1..4578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2135..2234)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2251..2270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2252..2270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2275..2291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2275..2291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2393..2412)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2404..3213
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2471..2490)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3360..3948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3849..3868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4102..4119
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4278..4305)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4397..4483)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4532..4554
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4546..4563
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4547..4563
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc agcagcaaag
      121 gtggccctga ccaagagggc cgatcctgcc gagctgcgca caatcttcct gaagtacgcc
      181 agcatcgaga agaacggcga gttctttatg tccccaaatg actttgtgac ccggtatctg
      241 aacatcttcg gcgagtctca gcccaatcct aagaccgtgg agctgctgag cggagtggtg
      301 gaccagacaa aggatggcct gatctccttc caggagtttg tggccttcga gtctgtgctg
      361 tgcgccccag acgccctgtt tatggtggcc tttcagctgt tcgataaggc cggcaagggc
      421 gaggtgacct tcgaggacgt gaagcaggtg tttggccaga ccacaatcca ccagcacatc
      481 cccttcaact gggatagcga gttcgtgcag ctgcactttg gcaaggagcg gaagagacac
      541 ctgacctacg ccgagtttac acagttcctg ctggagatcc agctggagca cgccaagcag
      601 gcctttgtgc agagagacaa tgcccggacc ggcagagtga cagccatcga ctttcgggat
      661 atcatggtga ccatcagacc ccacgtgctg acacctttcg tggaggagtg tctggtggca
      721 gcagcaggag gcaccacatc ccaccaggtg tcttttagct acttcaacgg ctttaattct
      781 ctgctgaaca atatggagct gatccggaag atctatagca ccctggccgg cacaagaaag
      841 gacgtggagg tgaccaagga ggagttcgtg ctggccgccc agaagtttgg ccaggtgaca
      901 cctatggagg tggacatcct gttccagctg gccgatctgt acgagccaag gggaaggatg
      961 accctggcag atatcgagag gatcgcccca ctggaggagg gcacactgcc ctttaacctg
     1021 gcagaggcac agaggcagaa ggcatccggc gactctgcca gacccgtgct gctgcaggtg
     1081 gcagagtctg cctacaggtt cggactgggc agcgtggcag gagcagtggg agcaaccgcc
     1141 gtgtatccta tcgatctggt gaagaccagg atgcagaacc agcgcagcac aggctccttt
     1201 gtgggcgagc tgatgtataa gaattccttc gactgcttta agaaggtgct gagatacgag
     1261 ggcttctttg gcctgtatag gggactgctg cctcagctgc tgggagtggc accagagaag
     1321 gccatcaagc tgaccgtgaa cgacttcgtg cgggataagt ttatgcacaa ggatggcagc
     1381 gtgcctctgg ccgccgagat cctggcagga ggatgtgcag gaggatccca ggtcatcttc
     1441 accaatccac tggagatcgt gaagatcagg ctgcaggtgg caggagagat caccacagga
     1501 ccaagggtgt ctgccctgag cgtggtgcgc gatctgggct tctttggcat ctacaagggc
     1561 gccaaggcct gctttctgag agacatcccc ttctccgcca tctactttcc ttgttatgcc
     1621 cacgtgaagg cctctttcgc caacgaggac ggacaggtgt ccccaggctc tctgctgctg
     1681 gcaggagcaa tcgcaggaat gccagcagca tctctggtga ccccagcaga tgtgatcaag
     1741 acaaggctgc aggtggcagc aagagcagga cagaccacat attccggcgt gatcgactgc
     1801 ttccggaaga tcctgagaga ggagggacca aaggccctgt ggaagggagc aggcgccagg
     1861 gtgttccgca gctcccctca gtttggcgtg accctgctga catacgagct gctgcagagg
     1921 tggttctata tcgattttgg cggcgtgaag cctatgggca gcgagccagt gcccaagtcc
     1981 cgcatcaacc tgcctgcacc aaatccagac cacgtgggag gatacaagct ggcagtggcc
     2041 accttcgccg gaatcgagaa taagtttggc ctgtatctgc ctctgttcaa gccatccgtg
     2101 tctacaagca aggccatcgg cggcggccca taagacccag ctttcttgta caaagttggc
     2161 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     2221 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     2281 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2341 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2401 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2461 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2521 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2581 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2641 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2701 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2761 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2821 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2881 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2941 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3001 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3061 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3121 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3181 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3241 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3301 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3361 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3421 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3481 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3541 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3601 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3661 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3721 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3781 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3841 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3901 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3961 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4021 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4081 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4141 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4201 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     4261 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     4321 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4381 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4441 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4501 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4561 agtcttaagc tcgggccc
//
