LOCUS       sequence-342385-       15471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is 
            TagRFP-tagged. LAT has three Tyr-Phe mutations..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Dine E, Reed EH, Toettcher JE
  TITLE     Positive feedback between the T cell kinase Zap70 and its substrate 
            LAT acts as a clustering-dependent signaling switch.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109280. doi: 
            10.1016/j.celrep.2021.109280.
  PUBMED    34161759
REFERENCE   2  (bases 1 to 15471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109280. doi"; pages: " 
            10.1016/j.celrep.2021.109280"
COMMENT     Sequence Label: pHR LAT-FFF/Zap70 iLID-Drop
FEATURES             Location/Qualifiers
     source          1..15471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             215..259
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    544..661
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        814..1221
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1287..2018
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             2094..2423
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     CDS             4314..4370
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5076..5513
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             5933..5944
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6204..7697
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    7829..8417
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7882..7902)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8300..8311)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8420..8436)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8421..8437)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8527..8760
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8785..8803)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8786..8803)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9093..9112)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9326..9345)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9445..9467
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9505..9523)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9591..9695
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9696..9764,9765..10556)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9914..9933)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10727..11315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11216..11235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11469..11486
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11556..11576)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11561..11890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11741..11876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          13024..13089
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13219..13239
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13664..13798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13701..13720)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13755..13774
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             13966..14599
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    14646..14771
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(15268..15471,1..30)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
       61 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
      121 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
      181 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
      241 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
      301 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
      361 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
      421 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
      481 cagatccatt cgattagtga acggatctcg acggtatcgc caaatggcag tattcatcca
      541 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      601 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
      661 tcgggtttat tacagggaca gcagagatcc agtttggatc gataagcttg atatcgaatt
      721 cctgcagccc cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
      781 gaccccacct gtaggtttgg caagctagct gcagtaacgc cattttgcaa ggcatggaaa
      841 aataccaaac caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg
      901 ttgggccaaa caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag
      961 atggtcaccg cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc
     1021 ccaaccctca gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga
     1081 aatgaccctg cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg
     1141 cttctgcttc ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc
     1201 cgacagactg agtcgcccgg gggggatctg gagctctcga gaattctcac gcgtcaagtg
     1261 gagcaaggca ggtggacagt ggatccatgg tgtctaaggg cgaagagctg attaaggaga
     1321 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     1381 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
     1441 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcagaa
     1501 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
     1561 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     1621 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat
     1681 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccaacacc gagatgctgt
     1741 accccgctga cggcggcctg gaaggcagaa ccgacatggc cctgaagctc gtgggcgggg
     1801 gccacctgat ctgcaacttc aagaccacat acagatccaa gaaacccgct aagaacctca
     1861 agatgcccgg cgtctactat gtggaccaca gactggaaag aatcaaggag gccgacaaag
     1921 agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac
     1981 tggggcacaa acttaatggc atggacgagc tgtacaagtc cggactcaga tctcgagctc
     2041 aagcttcgaa cgaattcggc attgatctga gcggcctgac cctgcaggaa ttcagctccc
     2101 cgaaacgccc taagctgctg cgtgaatatt acgattggct ggttgataac agctttaccc
     2161 catatctggt ggtggatgcc acatacctgg gcgtgaacgt gcccgtggag tatgtgaaag
     2221 acggtcagat cgtgctgaat ctgtctgcaa gtgcgaccgg caacctgcaa ctgacaaatg
     2281 attttatcca gttcaacgcc cgctttaagg gcgtgtctcg tgaactgtat atcccgatgg
     2341 gtgccgctct ggccatttac gctcgcgaga acggcgatgg tgtgatgttc gaaccagaag
     2401 aaatctatga cgagctgaat attggtagtg ctggtggtga attcatgatg ccagaccccg
     2461 cggcgcacct gcccttcttc tacggcagca tctcgcgtgc cgaggccgag gagcacctga
     2521 agctggcggg catggcggac gggctcttcc tgctgcgcca gtgcctgcgc tcgctgggcg
     2581 gctatgtgct gtcgctcgtg cacgatgtgc gcttccacca ctttcccatc gagcgccagc
     2641 tcaacggcac ctacgccatt gccggcggca aagcgcactg tggaccggca gagctctgcg
     2701 agttctactc gcgcgacccc gacgggctgc cctgcaacct gcgcaagccg tgcaaccggc
     2761 cgtcgggcct cgagccgcag ccgggggtct tcgactgcct gcgagacgcc atggtgcgtg
     2821 actacgtgcg ccagacgtgg aagctggagg gcgaggccct ggagcaggcc atcatcagcc
     2881 aggccccgca ggtggagaag ctcattgcta cgacggccca cgagcggatg ccctggtacc
     2941 acagcagcct gacgcgtgag gaggccgagc gcaaacttta ctctggggcg cagaccgacg
     3001 gcaagttcct gctgaggccg cggaaggagc agggcacata cgccctgtcc ctcatctatg
     3061 ggaagacggt gtaccactac ctcatcagcc aagacaaggc gggcaagtac tgcattcccg
     3121 agggcaccaa gtttgacacg ctctggcagc tggtggagta tctgaagctg aaggcggacg
     3181 ggctcatcta ctgcctgaag gaggcctgcc ccaacagcag tgccagcaac gcctcagggg
     3241 ctgctgctcc cacactccca gcccacccat ccacgttgac tcatcctcag agacgaatcg
     3301 acaccctcaa ctcagatgga tacacccctg agccagcacg cataacgtcc ccagacaaac
     3361 cgcggccgat gcccatggac acgagcgtgt atgagagccc ctacagcgac ccagaggagc
     3421 tcaaggacaa gaagctcttc ctgaagcgcg ataacctcct catagctgac attgaacttg
     3481 gctgcggcaa ctttggctca gtgcgccagg gcgtgtaccg catgcgcaag aagcagatcg
     3541 acgtggccat caaggtgctg aagcagggca cggagaaggc agacacggaa gagatgatgc
     3601 gcgaggcgca gatcatgcac cagctggaca acccctacat cgtgcggctc attggcgtct
     3661 gccaggccga ggccctcatg ctggtcatgg agatggctgg gggcgggccg ctgcacaagt
     3721 tcctggtcgg caagagggag gagatccctg tgagcaatgt ggccgagctg ctgcaccagg
     3781 tgtccatggg gatgaagtac ctggaggaga agaactttgt gcaccgtgac ctggcggccc
     3841 gcaacgtcct gctggttaac cggcactacg ccaagatcag cgactttggc ctctccaaag
     3901 cactgggtgc cgacgacagc tactacactg cccgctcagc agggaagtgg ccgctcaagt
     3961 ggtacgcacc cgaatgcatc aacttccgca agttctccag ccgcagcgat gtctggagct
     4021 atggggtcac catgtgggag gccttgtcct acggccagaa gccctacaag aagatgaaag
     4081 ggccggaggt catggccttc atcgagcagg gcaagcggat ggagtgccca ccagagtgtc
     4141 cacccgaact gtacgcactc atgagtgact gctggatcta caagtgggag gatcgccccg
     4201 acttcctgac cgtggagcag cgcatgcgag cctgttacta cagcctggcc agcaaggtgg
     4261 aagggccccc aggcagcaca cagaaggctg aggctgcctg tgccggaagc ggagctacta
     4321 acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct atggaggagg
     4381 ccatcctggt cccctgcgtg ctggggctcc tgctgctgcc catcctggcc atgttgatgg
     4441 ctctgtgtgt gcactgccat agactgcccg gttcttttga ttcaacatcc agcgacagcc
     4501 ttttccctag aggcatccag ttcaaacggc ctcatactgt ggccccgtgg cctcctgcat
     4561 ttcctcctgt cacatcattt ccacccctga gtcaaccaga tcttctccct atccccagat
     4621 caccccagcc tctcggtggc tcccacagaa ccccaagtag tcgaagagat tccgacgggg
     4681 cgaatagtgt agcgtctttt gagaacgagg aaccggcctg tgaggacgca gacgaggacg
     4741 aagacgattt ccacaaccca ggatacctgg tggtcttgcc cgactcaacg cctgcgacta
     4801 gcactgctgc cccaagcgcc cctgctctta gtaccccagg cataagagat tcagccttct
     4861 ccatggagtc aatcgatgat ttcgtcaacg tgccagagag cggcgagtct gccgaggcgt
     4921 cactggatgg gtccagggag ttcgtgaatg tcagccagga gttgcacccc ggggccgcca
     4981 aaactgaacc ggcggccctg tcctcccaag aggccgagga ggtggaagag gagggcgccc
     5041 ctgatttcga aaatttgcaa gaactgaacg agtttctggc aaccacactg gaacggatcg
     5101 agaaaaattt cgtgattact gatccgagac tgcctgacaa cccaatcatt tttgcgagcg
     5161 attccttcct gcagctgaca gaatattctc gggaagagat cctggggcgc aattgccgtt
     5221 ttctgcaggg acccgagaca gaccgtgcca ctgttcggaa aatcagagat gctattgaca
     5281 accagactga agtgaccgtt cagctgatca attataccaa gagcggcaag aagttctgga
     5341 acgtgttcca cctgcagccg atgcgcgatt ataagggcga cgtccagtac ttcattggcg
     5401 tgcagctgga tggcaccgaa cgtcttcatg gcgccgctga gcgtgaggcg gtctgcctga
     5461 tcaaaaagac agcctttcag attgctgagg cagcgaacga cgaaaattac tttggttcag
     5521 ggagcggatc cggctcaggg agtgcggccg caatggcctc aaacgattat acccaacaag
     5581 caacccaaag ctatggggcc taccccaccc agcccgggca gggctattcc cagcagagca
     5641 gtcagcccta cggacagcag agttacagtg gttatagcca gtccacggac acttcaggct
     5701 atggccagag cagctattct tcttatggcc agagccagaa cacaggctat ggaactcagt
     5761 caactcccca gggatatggc tcgactggcg gctatggcag tagccagagc tcccaatcgt
     5821 cttacgggca gcagtcctcc tatcctggct atggccagca gccagctccc agcagcacct
     5881 cgggaagtta cggtagcagt tctcagagca gcagctatgg gcagccccag agtgggagct
     5941 acagccagca gcctagctat ggtggacagc agcaaagcta tggacagcag caaagctata
     6001 atccccctca gggctatgga cagcagaacc agtacaacag cagcagtggt ggtggaggtg
     6061 gaggtggagg tggaggtaac tatggccaag atcaatcctc catgagtagt ggtggtggca
     6121 gtggtggcgg ttatggcaat caagaccaga gtggtggagg tggcagcggt ggctatggac
     6181 agcaggaccg tggaggtgga ggaatgaaga tggacaaaaa gactatagtt tggtttagaa
     6241 gagacctaag gattgaggat aatcctgcat tagcagcagc tgctcacgaa ggatctgttt
     6301 ttcctgtctt catttggtgt cctgaagaag aaggacagtt ttatcctgga agagcttcaa
     6361 gatggtggat gaaacaatca cttgctcact tatctcaatc cttgaaggct cttggatctg
     6421 acctcacttt aatcaaaacc cacaacacga tttcagcgat cttggattgt atccgcgtta
     6481 ccggtgctac aaaagtcgtc tttaaccacc tctatgatcc tgtttcgtta gttcgggacc
     6541 ataccgtaaa ggagaagctg gtggaacgtg ggatctctgt gcaaagctac aatggagatc
     6601 tattgtatga accgtgggag atatactgcg aaaagggcaa accttttacg agtttcaatt
     6661 cttactggaa gaaatgctta gatatgtcga ttgaatccgt tatgcttcct cctccttggc
     6721 ggttgatgcc aataactgca gcggctgaag cgatttgggc gtgttcgatt gaagaactag
     6781 ggctggagaa tgaggccgag aaaccgagca atgcgttgtt aactagagct tggtctccag
     6841 gatggagcaa tgctgataag ttactaaatg agttcatcga gaagcagttg atagattatg
     6901 caaagaacag caagaaagtt gttgggaatt ctacttcact actttctccg tatctccatt
     6961 tcggggaaat aagcgtcaga cacgttttcc agtgtgcccg gatgaaacaa attatatggg
     7021 caagagataa gaacagtgaa ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt
     7081 taagagagta ttctcggtat atatgtttca acttcccgtt tactcacgag caatcgttgt
     7141 tgagtcatct tcggtttttc ccttgggatg ctgatgttga taagttcaag gcctggagac
     7201 aaggcaggac cggttatccg ttggtggatg ccggaatgag agagctttgg gctaccggat
     7261 ggatgcataa cagaataaga gtgattgttt caagctttgc tgtgaagttt cttctccttc
     7321 catggaaatg gggaatgaag tatttctggg atacactttt ggatgctgat ttggaatgtg
     7381 acatccttgg ctggcagtat atctctggga gtatccccga tggccacgag cttgatcgct
     7441 tggacaatcc cgcgttacaa ggcgccaaat atgacccaga aggtgagtac ataaggcaat
     7501 ggcttcccga gcttgcgaga ttgccaactg aatggatcca tcatccatgg gacgctcctt
     7561 taaccgtact caaagcttct ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag
     7621 acatcgacac agctcgtgag ctactagcta aagctatttc aagaacccgt gaagcacaga
     7681 tcatgatcgg agcagcagcc cgggatccac cggtcgccac ctagcctgca ggcggccgca
     7741 actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg acctgcaggc
     7801 atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     7861 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7921 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7981 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8041 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8101 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8161 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8221 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8281 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8341 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     8401 ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg agctcggtac
     8461 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     8521 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     8581 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8641 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8701 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8761 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
     8821 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
     8881 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
     8941 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
     9001 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
     9061 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     9121 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     9181 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     9241 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     9301 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     9361 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     9421 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     9481 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     9541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9961 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10021 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10081 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    10141 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10201 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10261 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10321 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10381 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10441 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10501 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10561 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10741 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10801 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10861 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10921 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10981 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11041 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11101 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11161 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11221 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11281 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11341 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    11401 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    11461 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    11521 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
    11581 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    11641 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    11701 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
    11761 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    11821 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    11881 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
    11941 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
    12001 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
    12061 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
    12121 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
    12181 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
    12241 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
    12301 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
    12361 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
    12421 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
    12481 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
    12541 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
    12601 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
    12661 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
    12721 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
    12781 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
    12841 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
    12901 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
    12961 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
    13021 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
    13081 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
    13141 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
    13201 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
    13261 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
    13321 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
    13381 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
    13441 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
    13501 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
    13561 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    13621 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    13681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    13741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    13801 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    13861 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    13921 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
    13981 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
    14041 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
    14101 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
    14161 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
    14221 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
    14281 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
    14341 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
    14401 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    14461 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    14521 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    14581 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
    14641 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
    14701 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
    14761 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
    14821 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
    14881 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
    14941 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
    15001 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
    15061 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
    15121 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
    15181 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
    15241 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
    15301 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
    15361 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
    15421 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg t
//
