LOCUS       sequence-342408-        5372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of FLAG-tagged, codon-optimized, full-length SRSF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Campbell AE, Dyle MC, Albanese R, Matheny T, Sudheendran K, Cortazar
            MA, Forman T, Fu R, Gillen AE, Caruthers MH, Floor SN, Calviello L, 
            Jagannathan S
  TITLE     Compromised nonsense-mediated RNA decay results in truncated 
            RNA-binding protein production upon DUX4 expression.
  JOURNAL   Cell Rep. 2023 Jun 13;42(6):112642. doi: 
            10.1016/j.celrep.2023.112642.
  PUBMED    37314931
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-13"; volume: "42(6):112642. doi"; pages: " 
            10.1016/j.celrep.2023.112642"
COMMENT     Sequence Label: pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized
FEATURES             Location/Qualifiers
     source          1..5372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      237..373
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    525..596
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(601..647)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    665..713
                     /note="synthetic polyadenylation signal"
     promoter        750..1928
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          981..1919
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1876..1896
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2028..2051
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2612..2733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2623..2642)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2677..2696
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      2848..2894
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2924..3060
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3105..3176
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(3402..3990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3482..3501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(4736..4804,4805..5372,1..224)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4954..4973)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
       61 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
      121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
      181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaaccgatt ctaggtgcat
      241 tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga
      301 ttcagcaacg gatacggctt ccccaacttg cccacttcca tacgtgtcct ccttaccaga
      361 aatttatcct taagatcccg aatcgtttaa actcgactct ggctctatcg aatctccgtc
      421 gtttcgagct tacgcgaaca gccgtggcgc tcatttgctc gtcgggcatc gaatctcgtc
      481 agctatcgtc agcttacctt tttggcagcg atcgcggctc ccgacatctt ggaccattag
      541 ctccacaggt atcttcttcc ctctagtggt cataacagca gcttcagcta cctctcaatt
      601 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctatca atcgttgcgt
      661 tacacacaca aaaaaccaac acacatccat cttcgatgga tagcgatttt attatctaac
      721 tgctgatcga gtgtagccag atctcgtgag gctccggtgc ccgtcagtgg gcagagcgca
      781 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
      841 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      901 agggtggggg agaaccgtat ataagtgcac tagtcgccgt gaacgttctt tttcgcaacg
      961 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     1021 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     1081 atcccgagct tcgggttgga agtgggtggg agagttcgtg gccttgcgct taaggagccc
     1141 cttcgcctcg tgcttgagtt gtggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     1201 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     1261 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     1321 agcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     1381 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     1441 ctcaagctgc ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     1501 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     1561 gccctgctgc agggagcaca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     1621 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     1681 agtaccgggc gccgtccagg cacctcgatt agttctccag cttttggagt acgtcgtctt
     1741 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     1801 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     1861 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     1921 tgtcgtgaaa actaccccta aaagccaaaa gatctttgtc gatcctacca tccactcgac
     1981 acacccgcca gcggccgctg ccaagcttcc gagctctcga attcatggac tacaaagatg
     2041 acgacgataa aatgcacagg gactcctgcc cccttgactg caaggtttac gtgggcaacc
     2101 ttggaaataa tggtaacaag acagaactcg aacgggcctt cggctattat ggacccttgc
     2161 gctctgtatg ggtcgcgaga aatcctccag gctttgcatt cgtagaattt gaagatccac
     2221 gagacgcggc ggacgcagtt cgagagctgg acggtcgaac attgtgcgga tgtcgcgtga
     2281 gggttgaact ttctaacggt gaaaagcgga gccgcaatcg gggtccacca cccagttggg
     2341 gcagacgccc tcgcgacgat taccgccgca gaagtcctcc acctaggagg cgatcccccc
     2401 gacgacggtc attctctagg tctcggagcc gctcccttag ccgagaccga cggagggaga
     2461 gatccctgtc aagggagagg aatcacaaac cctcacgcag tttttcccgg agcaggagcc
     2521 gatctcgatc taatgaacga aagtagggat ccgcaggcct ctgctagctt gactgactga
     2581 gatacagcgt accttcagct cacagacatg ataagataca ttgatgagtt tggacaaacc
     2641 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     2701 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     2761 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     2821 ggtattggcc catctctatc ggtatcgtag cataacccct tggggcctct aaacgggtct
     2881 tgaggggttt tttgtgcccc tcgggccgga ttgctatcta ccggcattgg cgcagaaaaa
     2941 aatgcctgat gcgacgctgc gcgtcttata ctcccacata tgccagattc agcaacggat
     3001 acggcttccc caacttgccc acttccatac gtgtcctcct taccagaaat ttatccttaa
     3061 ggtcgtcagc tatcctgcag gcgatctctc gatttcgatc aagacattcc tttaatggtc
     3121 ttttctggac accactaggg gtcagaagta gttcatcaaa ctttcttccc tccctaatct
     3181 cattggttac cttgggctat cgaaacttaa ttaaccagtc aagtcagcta cttggcgaga
     3241 tcgacttgtc tgggtttcga ctacgctcag aattgcgtca gtcaagttcg atctggtcct
     3301 tgctattgca cccgttctcc gattacgagt ttcatttaaa tcatgtgagc aaaaggccag
     3361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3841 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4201 tgtctatttc gttcatccat agttgcattt aaatttccga actctccaag gccctcgtcg
     4261 gaaaatcttc aaacctttcg tccgatccat cttgcaggct acctctcgaa cgaactatcg
     4321 caagtctctt ggccggcctt gcgccttggc tattgcttgg cagcgcctat cgccaggtat
     4381 tactccaatc ccgaatatcc gagatcggga tcacccgaga gaagttcaac ctacatcctc
     4441 aatcccgatc tatccgagat ccgaggaata tcgaaatcgg ggcgcgcctg gtgtaccgag
     4501 aacgatcctc tcagtgcgag tctcgacgat ccatatcgtt gcttggcagt cagccagtcg
     4561 gaatccagct tgggacccag gaagtccaat cgtcagatat tgtactcaag cctggtcacg
     4621 gcagcgtacc gatctgttta aacctagata ttgatagtct gatcggtcaa cgtataatcg
     4681 agtcctagct tttgcaaaca tctatcaaga gacaggatca gcaggaggct ttcgcatgag
     4741 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4801 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcgcgagt
     4861 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4921 acgctttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4981 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5041 gtattcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5101 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgattggagg
     5161 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5221 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5281 agcaatggca acaaccttgc gtaaactatt aactggcgaa ctacttactc tagcttcccg
     5341 gcaacagttg atagactgga tggaggcgga ta
//
