LOCUS       sequence-34241-e         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1677-HQ858868
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             106..126
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             298..315
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccttgtt taactttaag
      121 aaggagccct tcaccatgaa caggatgacg gcgccgcacg gggggatgcc gccgccgccg
      181 atgccggcgg cgggggggct ggcgaggtac ggctcggcgc cggggtcgct gctggcgtcg
      241 atcgccgact cggtgatccg gggccgcggg gttggggttg tcgatcagct gcatcatcat
      301 cagcatcagc atcagcttcc gccgccgccg ccgccgcagc agcagcagat ggtggggagg
      361 tacttctccg cggagtcgtc ggggctcacc tcctgcgagt ccagctgccg gacgacgacg
      421 acgacgtcca cagcggcggc ggccgatgtc gggcggcacc cgctcgagcg cgcgtacggc
      481 ggctccggcg agatccacgt cgacgcctcc tccgccgccg tcccgctctt ccgccacagc
      541 agctcccccg ccggcctcct ctcccgcctc atggctgacc cgcacggcaa tggcatggcc
      601 gcgacgagag ggatgggcgg ctactccggc ggcggcggc
//
