LOCUS       sequence-342432-        6424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses recoded human Peg10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae
            RK, Koonin EV, Zhang F
  TITLE     Mammalian retrovirus-like protein PEG10 packages its own mRNA and 
            can be pseudotyped for mRNA delivery.
  JOURNAL   Science. 2021 Aug 20;373(6557):882-889. doi: 
            10.1126/science.abg6155.
  PUBMED    34413232
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-08-20- 20"; volume: "373"; 
            issue: "6557"; pages: "882-889"
COMMENT     Sequence Label: pCMV-HsPeg10rc3
FEATURES             Location/Qualifiers
     source          1..6424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          338..813
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             3115..3141
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3226..3620
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(3959..3976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4130..4718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4210..4229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4889..5680,5681..5749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5512..5531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5750..5854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5924..5943)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6045..6424
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
      301 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
      361 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
      421 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
      481 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
      541 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
      601 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
      661 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
      721 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
      781 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
      841 ctggcccatc actttggcaa agcacgtgag atctgaattc gccaccatgc tgggtcccga
      901 ctgcccacct cctcctcctc cccctccccc caacaacaac aacaacaaca actccaagca
      961 caccggccat aagagtgcgt gtgtccccaa catgaccgaa cgaagaaggg acgagctctc
     1021 tgaagagatc aacaacttaa gagagaaggt catgaagcag tcggaggaga acaacaacct
     1081 gcagagccag gtgcagaagc tcacagagga gaacaccacc cttcgagagc aagtggaacc
     1141 cacccctgag gatgaggatg atgacatcga gctccgcggt gctgcagcag ctgctgcccc
     1201 accccctcca atagaggaag agtgcccaga agacctccca gagaagttcg atggcaaccc
     1261 agacatgctg gctcctttca tggcccagtg ccagatcttc atggaaaaga gcaccaggga
     1321 tttctcagtt gatcgtgtcc gtgtctgctt cgtgacaagc atgatgaccg gccgtgctgc
     1381 ccgttgggcc tcagcaaagc tggagcgctc ccactacctg atgcacaact acccagcttt
     1441 catgatggaa atgaagcatg tctttgaaga ccctcagagg cgagaggttg ccaaacgcaa
     1501 gatcagacgc ctgcgccaag gcatggggtc tgtcatcgac tactccaatg ctttccagat
     1561 gattgcccag gacctggatt ggaacgagcc tgcgctgatt gaccagtacc acgagggcct
     1621 cagcgaccac attcaggagg agctctccca cctcgaggtc gccaagtcgc tgtctgctct
     1681 gattgggcag tgcattcaca ttgagagaag gctggccagg gctgctgcag ctcgcaagcc
     1741 acgctcgcca ccccgggcgc tggtgttgcc tcacattgca agccaccacc aggtagatcc
     1801 aaccgagccg gtgggaggtg cccgcatgcg cctgacgcag gaagaaaaag aaagacgcag
     1861 aaagctgaac ctgtgcctct actgtggaac aggaggtcac tacgctgaca attgtcctgc
     1921 caaggcctca aagtcttcgc cggcgggaaa ctccccggcc ccgctgtaga gggaccttca
     1981 gcgaccgggc cagaaataat aaggtcccca caagatgatg cctcatctcc acacttgcaa
     2041 gtgatgcttc agatacatct gccaggcagg cacacactct tcgtacgagc tatgattgat
     2101 tcaggggcat ctggtaactt catagatcac gagtatgtag ctcagaacgg aattcctttg
     2161 agaataaagg actggcccat cttggttgaa gctatcgatg ggaggcctat agcttccggt
     2221 cccgtggtgc atgaaacaca tgatcttatt gtcgatcttg gggatcaccg cgaagtcctc
     2281 tcatttgacg taacccagtc ccctttcttc cccgtggtcc tcggagtgcg gtggctgagt
     2341 actcacgatc ctaatattac atggagcacc cggtcaatcg tatttgatag tgagtactgt
     2401 aggtaccact gtcgaatgta tagtcctatt ccccctagtt tgcctccccc agctcctcaa
     2461 ccacccctgt actatcccgt ggacggatat agggtgtacc agcccgttcg atattactac
     2521 gtacaaaatg tttatacacc tgttgatgag cacgtctacc cagaccatag actcgtcgac
     2581 cctcacatcg aaatgattcc aggagcccat agtatacctt cagggcacgt atactctctg
     2641 tccgaacctg aaatggcagc tcttcgagat tttgtggcaa gaaatgtaaa agatgggcta
     2701 attactccaa cgattgcacc taatggagcc caagttctcc aggtgaagag ggggtggaaa
     2761 ctgcaagttt cttatgattg ccgagctcca aacaatttta ctatccagaa tcagtatcct
     2821 cgcctatcta ttccaaattt agaagaccaa gcacacctgg caacgtacac tgaattcgta
     2881 cctcaaatac ctggatacca aacatacccc acatatgccg cgtacccgac ctacccagta
     2941 ggattcgcct ggtacccagt gggacgagac ggacaaggaa gatcactata tgtacctgtg
     3001 atgatcactt ggaatccaca ctggtaccgc cagcctccgg taccacagta cccgccgcca
     3061 cagccgccgc ctccaccacc accaccgccg ccgcctccat cttacagtac cctgtatccg
     3121 tatgatgttc cggattatgc atgagaattc accccaccag tgcaggctgc ctatcagaaa
     3181 gtggtggctg gtgtggctaa tgccctggcc cacaagtatc actaagctcg ctttcttgct
     3241 gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac tgggggatat
     3301 tatgaagggc cttgagcatc tggattctgc ctaataaaaa acatttattt tcattgcaat
     3361 gatgtattta aattatttct gaatatttta ctaaaaaggg aatgtgggag gtcagtgcat
     3421 ttaaaacata aagaaatgaa gagctagttc aaaccttggg aaaatacact atatcttaaa
     3481 ctccatgaaa gaaggtgagg ctgcaaacag ctaatgcaca ttggcaacag cccctgatgc
     3541 ctatgcctta ttcatccctc agaaaaggat tcaagtagag gcttgatttg gaggttaaag
     3601 ttttgctatg ctgtatttta cattacttat tgttttagct gtcctcatga atgtcttttc
     3661 actacccatt tgcttatcct gcatctctca gccttgactc cactcagttc tcttgcttag
     3721 agataccacc tttcccctga agtgttcctt ccatgtttta cggcgagatg gtttctcctc
     3781 gcctggccac tcagccttag ttgtctctgt tgtcttatag aggtctactt gaagaaggaa
     3841 aaacaggggg catggtttga ctgtcctgtg agcccttctt ccctgcctcc cccactcaca
     3901 gtgacccgga atccctcgac atggcagtct agcactagtg cggccgcaga tctgcttcct
     3961 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4021 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4081 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4141 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4201 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4261 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4321 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4381 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4441 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4501 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4561 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4621 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4681 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4741 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4801 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4861 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4921 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4981 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5041 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5101 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5161 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5221 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5281 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5341 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5401 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5461 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5521 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5581 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5641 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5701 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5761 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5821 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5881 acgtggatcc cctgaggggg cccccatggg ctagaggatc cggcctcggc ctctgcataa
     5941 ataaaaaaaa ttagtcagcc atgagcttgg cccattgcat acgttgtatc catatcataa
     6001 tatgtacatt tatattggct catgtccaac attaccgcca tgttgacatt gattattgac
     6061 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     6121 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     6181 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     6241 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     6301 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     6361 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     6421 catg
//
