LOCUS       sequence-342438-        3059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor plasmid for SapTrap assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Kroll JR, Remmelzwaal S, Boxem M
  TITLE     CeLINC, a fluorescence-based protein-protein interaction assay in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 
            10.1093/genetics/iyab163.
  PUBMED    34849800
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-12-10"; volume: "219(4). pii: 6380436. doi"; pages: " 
            10.1093/genetics/iyab163"
COMMENT     Sequence Label: pJRK151
FEATURES             Location/Qualifiers
     source          1..3059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(316..335)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             327..1142
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(400..419)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1346..1368
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1360..1377
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1361..1377
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1832..1848)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1832..1848)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1845..1867)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1856..1872
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1880..1886,1887..1904,1905..1910))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1925..1946
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2247..2264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2418..3006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2498..2517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat
       61 ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg
      121 tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct
      181 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc
      241 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
      301 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga
      361 agccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      421 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
      481 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
      541 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
      601 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
      661 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
      721 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
      781 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
      841 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
      901 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
      961 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     1021 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1081 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     1141 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     1201 gcggctttgt tgaataaatc gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1261 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1321 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     1381 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct gctcttcgct
     1441 taccttaaaa ttcaaaaatt aatttcagat cagtcataac caagaaaaca aaataaacgt
     1501 taggacagaa aattcaaatt aattaaaaat tatgctgaat aagaaaatta catgcaaagc
     1561 cttattttcc tcatagaaac agttgaataa catatttttt aattgaaaac atagcaaaat
     1621 tcgtggtaaa atttgtcaaa accttggaaa aaatcgaaaa aagaacattt cccgatgcat
     1681 tttcctattt ctcgttgccg acaacgtata aacgttgaat actcagtcaa cgaatttggc
     1741 tgctggcttg gactctcgca cggaagagac gcgtgtgtag atttacgacg aaagcaaaaa
     1801 tccacgaaga gcagctcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     1861 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1921 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1981 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2041 gtattggcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
     2101 ccgttccgtg gcaaagcaaa agttcaaaat cagtaaccgt cagtgccgat aagttcaaag
     2161 ttaaacctgg tgttgatacc aacattgaaa cgttgatcga aaacgcgctg aaaaacgctg
     2221 ctgaatgtgc gagcttcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2281 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2341 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2401 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2461 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2521 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2581 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2641 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2701 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2761 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2821 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2881 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2941 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3001 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa ctccgtcga
//
