LOCUS       sequence-342440-        6339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor plasmid for SapTrap assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Kroll JR, Remmelzwaal S, Boxem M
  TITLE     CeLINC, a fluorescence-based protein-protein interaction assay in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 
            10.1093/genetics/iyab163.
  PUBMED    34849800
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-12-10"; volume: "219(4). pii: 6380436. doi"; pages: " 
            10.1093/genetics/iyab163"
COMMENT     Sequence Label: pJRK143
FEATURES             Location/Qualifiers
     source          1..6339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(316..335)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             327..1142
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(400..419)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1346..1368
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1360..1377
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1361..1377
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2733..2744)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5112..5128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5112..5128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5125..5147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5136..5152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5160..5166,5167..5184,5185..5190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5205..5226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5527..5544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5698..6286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5778..5797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat
       61 ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg
      121 tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct
      181 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc
      241 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
      301 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga
      361 agccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      421 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
      481 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
      541 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
      601 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
      661 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
      721 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
      781 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
      841 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
      901 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
      961 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     1021 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1081 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     1141 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     1201 gcggctttgt tgaataaatc gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1261 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1321 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     1381 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgaggt gctcttcgct
     1441 ttctataatc tattttctag tttctatttt attagaatgc caagaaaatc aagcatttat
     1501 acatcaaaac ttatcaatga aaacagtggc acctaaaaca tcaagcagtt taaaaattca
     1561 aaaattcaaa aatgcttcga gaagtgtatt ttcttaaata ctgcctttaa aattaaaact
     1621 atttgaagac aattttcaga cgaagatatt acaattaaca ttttcttgct cattccaaaa
     1681 attaactgca aaaattaatt aaaataaccc ataaaataag aacacaaaga tgttaatgcc
     1741 atagtaactt agaagatatc agtgaaaaca gtggcacctc gatagctgtt caacgataag
     1801 actaaaaatg attgggaagt atagataggc tgattttatc aaaaagaaaa ttgtcaatga
     1861 aggcacaaag cgtattggtt tgaaaagaaa gatggtgtgc acaccgagct cggtgtgcat
     1921 gatggctggc tagcgattat ggcgttagtg tcgactattc ttatcagttt cagccccgac
     1981 ctctgtcgct catatacgca gacaaccacc cacttccagc caaacatggg actcgggtcc
     2041 gcccatcatt gcatgtacgg tagttggtaa ttgaaaaaag tggaatgagg aagaaaatat
     2101 tggtttcaaa gtagcgttta taataactac aaaaaaatca actgaggaga caataagtag
     2161 ataaaacagg cgatcagact tttgatggat acatatatcc aattttgcaa acaaacttga
     2221 aaattaaatt gagtttttgc gttttcactg aaactttcaa acaagttgca atgcagctat
     2281 taatactgca catagtaata gtcttgcttt aatttattgc cagaacagat gtatctagtt
     2341 aaaatgttga taaattacta tccttcacag agtgaaacaa aatcaaaagc tttcaagaat
     2401 ttatggtaat tttttttaat ttgataaggc atcattgtca aaacttgaat aatttgtcaa
     2461 aagctagata ttcaaaaatt agtattgaac ctcaattacc ctacaaagtt tatagtgctc
     2521 accaattgga aataagtttt gaatacaata ctaatggaga aaatacggca aactatacat
     2581 ttttaacttt tcactcatta gttttcatat aatttttaaa ctgtgatgtc acaccataat
     2641 tttaaagaca agaaaacata caaatacata taattatcaa taaatgatat tattataaaa
     2701 taaaaaacgc atatttgtga gatatgaaaa gtacgccctt ctatgttttt tttcaagaat
     2761 accggatcaa agggtacgct ttttgttaca cagtgtgttt tacggaaaca tattcaacat
     2821 ttggaggaca gttctgtctc caaagctgac cagttcaaca ttcttataaa caaaacgtga
     2881 tgcgaccttc aagttgtctg gaacttttgt gattcgggca ggataaggag tatgaagaat
     2941 acaataatga aaaataaaac aaaaaaagat aacctgtaga aaaattgata attttcagtt
     3001 tttttcgata atcagccaca cacgcacata atattacttc acaccccaca atatcagata
     3061 ataatcaaaa atgataaaat atatctttat acatcgataa cacaatcaaa ctagtgataa
     3121 attggtattt caagatttca accatatgct ctccgaactg tatactacag taatattatt
     3181 gggtacaagg tctaaaaaca ttagcatgtt gtaaaaccac ttcttttgct ctcatttctc
     3241 aatgtttgcg ctcagtaagt gagcttcttt tgaaatgaag tacggtcgcc ttatcagttg
     3301 catgacgttt gagccgattc tttccttttt gtcttcaaat ttcctattca gccagcaaac
     3361 tcggggaagt caaggatgaa cgtttaatag agaaaaacta tgggtgttct gaaacaatca
     3421 gtaaaaaaat tatcaggaca aaatggcaac attatcgttg agaatgcgtc ctcgtatcgc
     3481 cgtagctcag aagtagaaat caattgaaga taacattatc aattctgtat aaaaagaaaa
     3541 tacaggtttt gatatcaaac cttaacacat ttaacatttg gtcattatat tccataatag
     3601 tgtcatcaat ttagttctgc aaaaacacat ctgctcacac tactctctat atccgaaatg
     3661 acacaattag ctttcgaagt tatttatttg gtgtcgatag ttgtatatga gtgttgtgac
     3721 tccgtttata aaccgttttt aacagtcggg aataaattta tatttttcac tttaaaatta
     3781 atctggtatc ttttttttaa tttggttttt taacgggggg tttgaagttc aaagaaaatc
     3841 aaaatcaaaa ttctttattt agaaagtttg ttaagtaatt gaaggttttt tggagattct
     3901 aaaatcagct gtataagtat ttctaaacag cgtatctttc aaaaaagaaa aacttttttg
     3961 acgtcaaagg gttagaaaat cactttcaca taatttctgg atgtcaaacg gtccgagagc
     4021 ttttttaata cgtgaaacat tccatcacgt cttcatttta gttttttttt ttttatttat
     4081 ataattcaat ttatgtttct taaaactgta aatttatatt tattaagtca gtacaaaaaa
     4141 ttctgaattg gtcaattagg aactgtgaac gtttcagagt agatttgtga tgacggttct
     4201 tttttgttta ttaaacattt catttatgaa tatcacattt tcccatcagt tctttttttt
     4261 tcaaattcat gatcacagga aacattttat aactgtcaga atacttggtg cgagccatca
     4321 cacgaaggca tgcaaaaact tgattgaaca ttgatttttt tgtactttca gggatattca
     4381 ggtaaaaact gaacattggt tagcggtgat ttcttctggt gccggtaaag atgtatataa
     4441 ctttatttgt gttcaaaaat aaccatggaa gtatgttagc aagtacattt ttaacgtttt
     4501 caatctgatt atttttcaat tagtatgact catcgagcat ttttagtttt tttttcagtt
     4561 ttagaatcag aaatctacaa aaaaagcact ctactcaaga tcagtcattt tctgatgatt
     4621 tattgttttc tcgccactaa tttcctactt tgatcatcat tttttatcat taacattttt
     4681 gaatttatat tattagatta taacttgatg aatgtttgca actgtattct taatgctcct
     4741 gttttttcgt cccacataag tctctaaata ctatttctaa acatatttga tactttccaa
     4801 gcatttattg ggtctttcac attcgatctg ttatcagtag ttatgcacac gacaagacac
     4861 tagatggtac aatcgagagg caataaactg tttacacatt cttttcagtt cgtcttattc
     4921 tctctcactt tctcattgtc tttattagtt gagaaaatgt acgggttggg ggagaactaa
     4981 gagcagaatg ggaaaaaagg tgggtaatag gattataata ataatagtta cagagtcagg
     5041 aattggttat caagaattgg agttcataca tttcgaaatt accacgaaga gcagctcgaa
     5101 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5161 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5221 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5281 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattggcga acttttgctg
     5341 agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa
     5401 agttcaaaat cagtaaccgt cagtgccgat aagttcaaag ttaaacctgg tgttgatacc
     5461 aacattgaaa cgttgatcga aaacgcgctg aaaaacgctg ctgaatgtgc gagcttcttc
     5521 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5581 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5641 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5701 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5761 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5821 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5881 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5941 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6001 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6061 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6121 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6181 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6301 cttttctacg gggtctgacg ctcagtggaa ctccgtcga
//
