LOCUS       sequence-342443-        3279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor plasmid for SapTrap assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Kroll JR, Remmelzwaal S, Boxem M
  TITLE     CeLINC, a fluorescence-based protein-protein interaction assay in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 
            10.1093/genetics/iyab163.
  PUBMED    34849800
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-12-10"; volume: "219(4). pii: 6380436. doi"; pages: " 
            10.1093/genetics/iyab163"
COMMENT     Sequence Label: pJRK1
FEATURES             Location/Qualifiers
     source          1..3279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     116..133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    434..455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(470..475,476..493,494..500)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    508..524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     513..535
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     532..548
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     532..548
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1223..1240)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1223..1239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1232..1254)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(1458..2273)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2181..2200
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2265..2284
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2653..3241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3142..3161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
       61 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      121 gtcagtgagc gaggaagcgg aagaagctcg cacattcagc agcgtttttc agcgcgtttt
      181 cgatcaacgt ttcaatgttg gtatcaacac caggtttaac tttgaactta tcggcactga
      241 cggttactga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc
      301 gtgatctgat ccttcaactc agcaaaagtt cgccaatacg caaaccgcct ctccccgcgc
      361 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      421 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      481 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      541 gctatgacat gattacgaat tcgagctgct cttcgtggat ttttgctttc gtcgtaaatc
      601 tacacacgcg tctcttccgt gcgagagtcc aagccagcag ccaaattcgt tgactgagta
      661 ttcaacgttt atacgttgtc ggcaacgaga aataggaaaa tgcatcggga aatgttcttt
      721 tttcgatttt ttccaaggtt ttgacaaatt ttaccacgaa ttttgctatg ttttcaatta
      781 aaaaatatgt tattcaactg tttctatgag gaaaataagg ctttgcatgt aattttctta
      841 ttcagcataa tttttaatta atttgaattt tctgtcctaa cgtttatttt gttttcttgg
      901 ttatgactga tctgaaatta atttttgaat tttaaggtaa tatgcgaaga gcagctgctc
      961 ttcgtggatt tttgctttcg tggtaaaatt tgtcaaaacc ttggaaaaaa tcgaaaaaag
     1021 aacatttccc gatgcatttt cctatttctc gttgccgaca acgtataaac gttgaatact
     1081 cagtcaacga atttggctgc tggcttggac tctcgcacgg aagagacgcg tgtgtagatt
     1141 tacgacgaaa gcaaaaatcc acgaagagca gctcggtacc cggggatcct ctagagtcga
     1201 cctgcaggca tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1261 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1321 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgcg
     1381 atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac
     1441 caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     1501 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     1561 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     1621 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     1681 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     1741 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     1801 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     1861 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     1921 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     1981 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     2041 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     2101 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     2161 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     2221 ttggaattta atcgcggctt cgagcaagac gtttcccgtt gaatatggct cataacaccc
     2281 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     2341 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     2401 tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca
     2461 aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc
     2521 tccctcactt tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct
     2581 tcttcacgag gcagacctct cgacggagtt ccactgagcg tcagaccccg tagaaaagat
     2641 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2701 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2761 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2821 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2881 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2941 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3001 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3061 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3241 aaacgccagc aacgcggcct ttttacggtt cctggcctt
//
