LOCUS       sequence-342445-        4870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CeLINC plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Kroll JR, Remmelzwaal S, Boxem M
  TITLE     CeLINC, a fluorescence-based protein-protein interaction assay in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 
            10.1093/genetics/iyab163.
  PUBMED    34849800
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-12-10"; volume: "219(4). pii: 6380436. doi"; pages: " 
            10.1093/genetics/iyab163"
COMMENT     Sequence Label: pJRK136
FEATURES             Location/Qualifiers
     source          1..4870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..60
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    170..191
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(206..211,212..229,230..236)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    244..260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     249..271
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     268..284
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     268..284
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     303..323
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        305..323
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             1354..1377
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2326..2342)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2327..2343)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2367..2386)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2368..2386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2396..2413)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2396..2412)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2405..2427)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2554..3009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2641..2660)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2851..2872
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3035..3139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3140..3208,3209..4000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3358..3377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4171..4759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4660..4679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
       61 gaagcggccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      121 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      181 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      241 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      301 gcgcaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct
      361 ctagaagggc tgcaggaatt cgattggatt tttgctttcg tcgtaaatct acacacgcgt
      421 ctcttccgtg cgagagtcca agccagcagc caaattcgtt gactgagtat tcaacgttta
      481 tacgttgtcg gcaacgagaa ataggaaaat gcatcgggaa atgttctttt ttcgattttt
      541 tccaaggttt tgacaaattt taccacgaat tttgctatgt tttcaattaa aaaatatgtt
      601 attcaactgt ttctatgagg aaaataaggc tttgcatgta attttcttat tcagcataat
      661 ttttaattaa tttgaatttt ctgtcctaac gtttattttg ttttcttggt tatgactgat
      721 ctgaaattaa tttttgaatt ttaaggtaag atgaacggag ctatcggagg agaccttctc
      781 ctcaacttcc cagacatgtc cgtcctcgag cgtcaacgtg ctcacctcaa gtacctcaac
      841 ccaaccttcg attccccact cgccggattc ttcgccgatt cttccatgat caccggagga
      901 gagatggact cctacctttc caccgccgga ctcaatcttc caatgatgta cggagagacc
      961 accgtcgagg gagattcccg tctctccatc tccccagaga ccacccttgg aactggaaac
     1021 ttcaaggccg ccaagttcga taccgagact aaggattgca acgaggccgc caagaagatg
     1081 accatgaacc gtgatgacct cgtcgaagaa ggagaagagg agaagtccaa gatcaccgag
     1141 caaaacaacg gatccaccaa gtccatcaag aagatgaagc acaaggccaa gaaggaagag
     1201 aacaacttct ctaacgattc ctccaaggtc accaaggagc tcgagaagac cgattacatc
     1261 gattacaagg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
     1321 gatcacgatg gagactacaa ggaccacgat atcgactaca aggacgatga cgataaagga
     1381 tccggaggtg gaggtggcgg atccgggacg ggaggaaagt ccggaggaaa caagaagaac
     1441 gatggagtca aggagtcttc tgagtccacc aacaccacca tcgaggacga ggacaccaag
     1501 gtccgcaagc aagaaatcat caaggtcacc gagcaactca tcgaggccat ctccaacgga
     1561 gacttcgagt cctacaccaa ggtaagttta aacatatata tactaactaa ccctgattat
     1621 ttaaattttc agatgtgcga cccaggaatg accgccttcg agccagaggc cctcggaaac
     1681 ctcgtcgagg gactcgactt ccaccgtttc tacttcgaaa acctctggtc ccgtaactcc
     1741 aagccagtcc acaccaccat cctcaaccca cacatccacc tcatgggaga cgagtccgcc
     1801 tgcatcgcct acatccgcat cactcaatac ctcgatgccg gaggaatccc acgcaccgcc
     1861 caatccgagg agacccgcgt ctggcaccgc cgtgacggaa agtggcaaat cgtccacttc
     1921 caccgttctg gagccccatc cgtcctccca cactgctaat ccaattactc ttcaacatcc
     1981 ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct
     2041 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     2101 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     2161 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac
     2221 ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     2281 gttacgtttc agtttatgac cgcaattttt agtaatcaag cttatcgata ccgtcgacct
     2341 cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     2401 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2461 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2521 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     2581 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2641 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2701 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2761 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     2821 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     2881 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     2941 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     3001 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3061 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3301 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3361 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4021 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4081 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4861 taccgccttt
//
