LOCUS       sequence-342448-        6016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse syncytin B in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae
            RK, Koonin EV, Zhang F
  TITLE     Mammalian retrovirus-like protein PEG10 packages its own mRNA and 
            can be pseudotyped for mRNA delivery.
  JOURNAL   Science. 2021 Aug 20;373(6557):882-889. doi: 
            10.1126/science.abg6155.
  PUBMED    34413232
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-08-20- 20"; volume: "373"; 
            issue: "6557"; pages: "882-889"
COMMENT     Sequence Label: pCMV-SynB
FEATURES             Location/Qualifiers
     source          1..6016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          338..813
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     polyA_signal    2818..3212
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(3551..3568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3722..4310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3802..3821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4481..5272,5273..5341))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5104..5123
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5342..5446)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5516..5535)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        5637..6016
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
      301 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
      361 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
      421 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
      481 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
      541 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
      601 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
      661 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
      721 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
      781 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
      841 ctggcccatc actttggcaa agcacgtgag atctgaatta tgacaggctt ttgggtcctc
      901 tgtttcgtcc ttttcccctc ctccttatcc tatccggaaa gctggatgcc ccttgtaaac
      961 ctcactcacc acatcctacg tgataccaac tcttccctgt tttccaactg ttgggtctgc
     1021 ttgtctaccc aaacccagcg gtccttagca gtcccagccc ctctgtccat ttggacagat
     1081 acacccatga agcttcatct tacctactca gtcaggccct tctctggctc cttttccatt
     1141 agcgacattg aaagacgcct ccgtctcttc cgcccactga ctgcctccta ttctttccac
     1201 aatcctgaca gaagggcgat tgcttttctt caactcgtca gctcaacagg catatttcgg
     1261 atcatcaccc ggataacctc tgtgatatat ccccataagg accgtttctt cgaatctgcc
     1321 caacgccctc tctggggacc actctttact gagaccgtgc tcaggtcgca ggccccactc
     1381 tgcatatctc gctttttcaa ggtctcagca tatgccactt ttgtaggcaa cctctctgcc
     1441 tctctctgca actacaccat gcatatttca ccttctacca gtcatgaaaa cctagatctt
     1501 tccaccaccc atacgttcaa acaggcaatg aaaagaccgg atgccaaatg gaaaaacccg
     1561 ctccgttttt ccgggccccc ctccctcatc ttctcgaagc cggcttacta tccctgccca
     1621 acagacatca aacactgcca tacctctccg gccactccct ggatgcactg tcctcaggct
     1681 cccttcggca cctgctataa cctcacttta tttgaaccag acaactcaac ccaccctgtt
     1741 accatgtcag tgaaccctac ccacttcaag gtcaaactcc aggggcacag agacccctat
     1801 ccgctctccc attaccagcc cctcacggga gctgccctgt ctggacaata ttcagtctgg
     1861 gagaacgaga tcactgtcca agaaaactgg gacatcacct ccaacatttt ctcacatctt
     1921 ctcagcttct cgtacgcctt ctgcctcaac tcttcaggcg ttttcttcct ctgcggaaca
     1981 tcgacttaca tctgcctccc agccaattgg tccggtgtct gtaccctggt cttccaatac
     2041 ccggatattg aacttctccc caataaccaa acggtgcctg ttcccctttt tgcttcagtt
     2101 ctttcctcag actcagttct tcgcccaaag aggtcccctc acctctttcc cttccttgca
     2161 ggcctgggta tctcttctgc ccttggtacg gggatagctg gcttggccac ctcgactctc
     2221 tatttccaac agctttctaa ggttctttcc gaaaccttgg aagaaatagc tgcctctatc
     2281 actaccctcc agaaccaaat agactcgctc gcaggtgttg ttctacaaaa ccgccgagct
     2341 ctggacctca tcactgctga gaaagggggc acctgtctct tcctccagga agagtgctgc
     2401 ttctacgtaa accagtctgg aatagtccgg gacgcggcaa ggaaactcca agaacgagca
     2461 tctgaactcg gccagcattc tgactcttgg ggacagtggc ctgaccttgg acgttggttg
     2521 ccctggctga ctccctttct gggacctctt ctcttcctct tcttcctact gacatttggg
     2581 tcttgtcttc tgaactgcct aacccgtttt gtgtcccaga gacttggctc ctttgttcaa
     2641 gacactgcca aaaggcatgt ggacagcatc ctccaaaatt tccaatataa aaaactgccc
     2701 caagactccc cagatgagga caccattcct acataagaat tcaccccacc agtgcaggct
     2761 gcctatcaga aagtggtggc tggtgtggct aatgccctgg cccacaagta tcactaagct
     2821 cgctttcttg ctgtccaatt tctattaaag gttcctttgt tccctaagtc caactactaa
     2881 actgggggat attatgaagg gccttgagca tctggattct gcctaataaa aaacatttat
     2941 tttcattgca atgatgtatt taaattattt ctgaatattt tactaaaaag ggaatgtggg
     3001 aggtcagtgc atttaaaaca taaagaaatg aagagctagt tcaaaccttg ggaaaataca
     3061 ctatatctta aactccatga aagaaggtga ggctgcaaac agctaatgca cattggcaac
     3121 agcccctgat gcctatgcct tattcatccc tcagaaaagg attcaagtag aggcttgatt
     3181 tggaggttaa agttttgcta tgctgtattt tacattactt attgttttag ctgtcctcat
     3241 gaatgtcttt tcactaccca tttgcttatc ctgcatctct cagccttgac tccactcagt
     3301 tctcttgctt agagatacca cctttcccct gaagtgttcc ttccatgttt tacggcgaga
     3361 tggtttctcc tcgcctggcc actcagcctt agttgtctct gttgtcttat agaggtctac
     3421 ttgaagaagg aaaaacaggg ggcatggttt gactgtcctg tgagcccttc ttccctgcct
     3481 cccccactca cagtgacccg gaatccctcg acatggcagt ctagcactag tgcggccgca
     3541 gatctgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3601 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3721 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3841 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3901 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3961 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4021 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4081 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4141 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4201 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4501 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4561 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4621 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4681 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4741 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4801 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4861 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4921 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4981 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5041 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5101 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5161 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     5221 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5281 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5341 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5401 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5461 aagtgccacc tgacgtggat cccctgaggg ggcccccatg ggctagagga tccggcctcg
     5521 gcctctgcat aaataaaaaa aattagtcag ccatgagctt ggcccattgc atacgttgta
     5581 tccatatcat aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca
     5641 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     5701 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     5761 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     5821 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     5881 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     5941 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     6001 catcgctatt accatg
//
