LOCUS       sequence-34247-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1751-HQ858874
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             106..126
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(548..647)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(664..683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(665..683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(688..704)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(688..704)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccttgtt taactttaag
      121 aaggagccct tcaccatggg caaggccgac gccgacgccg agttcaaggc cgccggcaag
      181 cgcaagaagg ccggaggcgc cggcaagccc aagcgcggcc tcaccccgtt cttcgccttc
      241 ctggctgagt tcaggccgca gtacatggag aagcacccaa acaccaaggg cgtcgcagcg
      301 gtgaccaagg ctgccgggga gaagtggcgc gctatgtcgg acgaggagaa agcgcaatat
      361 gggggcaaga agccagatgg cgagagcaag cccgcggccg ctagcaagaa gaaggagagc
      421 actagctcta agaaggctaa aactgatggt gcggaacagg agggagaagg ttctgacaag
      481 tcgaagtctg atgttgagga tgatgagaat gatggcagtg gcgaggacga gaagggtggg
      541 cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
      601 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
      661 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
      721 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
      781 ctgtctgctt acataaa
//
