LOCUS       sequence-34248-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1752-HQ858875
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             106..126
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(734..758)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccttgtt taactttaag
      121 aaggagccct tcaccatggc ggcttccgta ggactgaacc ctgaagcttt cttcttcagc
      181 aactcctact cctactcctc atcccctttc atggccagct acacgccgga gttctcggcc
      241 gccgctatcg acgccaactt attctccggc gagctcgatt tcgactgctc tctcccagct
      301 ccggcccagg agtacccgga aaatgaaaac actatgatga ggtacgagag cgaggagaag
      361 atgagggcga gggtgaacgg gaggatcggg ttcaggacga ggtcggaggt ggagattctt
      421 gatgatggct tcaagtggag gaagtacggc aagaaagctg tcaagaacag cccaaatcca
      481 agaaactact accggtgctc gacggagggg tgcaacgtga agaagcgagt ggagagagac
      541 cgggaggacc accgctacgt catcaccacc tacgacggcg tccacaacca cgcgagcccc
      601 gccgccgccg ccgcggcgct gcagtacgcc gccgccgccg gcgactacta cagcccgccg
      661 ctcagcagcg ccgtctcgcc gccggccgcc tattcggcag gcggctcgct gctcttcaag
      721 ggtgggcgcg ccgacccagc tttcttgtac aaagttggca ttataa
//
