LOCUS       sequence-342481-         483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Kazachstania naganishii (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK012
FEATURES             Location/Qualifiers
     source          1..483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(422..439)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 atgaaatttt ctactatttt atctgctgct gctttagctg cttctgcttt tgctgctcca
       61 attgaacaac cagaacaaga tttgaattct actactattc cagctgaagc cattatttct
      121 tacttggatt tagaaggtga taaagatatt gctgttgttc cattttctaa tgctactgat
      181 tctggtttat tatttgttaa tactacaatt ttagctcaag ctaataaaga agctggtact
      241 ccattacaaa aaagagaagc agacgctgat gccaatgcct ggcactggtt gagattatct
      301 tatggccaac ctatttacaa aagggatgct aacgcagatg cagatgctga cgcagatgca
      361 tggcattggc taaggttaag ttatggtcaa ccaatataca agagagatgc aaatgcagat
      421 gctgatgctg atgctgatgc ttggcattgg ttaagattgt catatggtca accaatttat
      481 taa
//
