LOCUS       sequence-342485-         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Lachancea mirantina (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK014
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             492..503
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgaaattaa ctgttttttg gactgctttt ctatcattct tgactacttt tattgttact
       61 gcaagtgctg ctccagttgc agattcagca aatgatggtt ctgttaatat tccagctgaa
      121 tctattattg gttttttgga tttgtctgat tctaaagatg ctactttggt tcctgtttct
      181 aatggtactc attctggttt cttattttta aattctacaa ttttatctca agcttctact
      241 gaaagtttga aaaaaagatc tgctcaacca tggagatggt tgtctttaag agctggtgaa
      301 gctttgcata aaagagaagc tgatgctgaa gctgatccat ggcaatggtt atctttaaga
      361 gctggtgaag cattacataa gagagaggca gaagctgcac cttggtcatg gttatcatta
      421 agagcaggtg aagctttaca taaaagaaat gctaatgcag aagctgaggc agcaccatgg
      481 aggtggctgt cattgagggc aggcgaagcc ttacacaaaa gggaagccga tgcagctcct
      541 tggagatggc tatctttgag agctggtgag gctttacatt aa
//
