LOCUS       sequence-34250-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1755-HQ858877
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             106..126
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccttgtt taactttaag
      121 aaggagccct tcaccatggc ggcggaggcc ggcagcgcgg ccgccggcgc cgcctacgag
      181 gaggagcgcc ggaagcgggt cctcgagaac ctcaagcacc tcgaggatct gggcataaag
      241 aagatgtcca agagcttgct ggaggcggcg agattgcaga agagcactcg tgccagcccc
      301 aagccgcgga agaagtttga ggttggggcc actgaagtga ggcgttcatc gagggcgcgc
      361 aactcggttt cgtacaaaga gaattttgat gaactcaaca gttttctatg ccgtagaagg
      421 ggcagtagga ttaggagcac agagcagggg agggattaca ccggaagagt tgcctcttat
      481 gaacagcaac aacgtgcttt caaaaaagct gagagacttc aaaatagtct agaccctgag
      541 aacccgtcgt ttgttaagac catggttcga tcacatgtgt ccagctgctt ttggcttggt
      601 cttcgaacta gattctgcaa attgcatctg cctcccaaag agtataagat ggtcttggag
      661 gatgaagagg gtggtgaatt tgattctgtc tacattggga accgtacagg cctaagtggt
      721 ggatggagag gttttgcgat gcaccacaac ttggaagatg gtgattcatt ggtctttgaa
      781 ttggctgaac cagacagatt taagatctac atcataaaag ctgttgatga
//
