LOCUS       sequence-34251-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1858-HQ858878
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             106..126
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             271..294
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccttgtt taactttaag
      121 aaggagtcct tcaccatgga cgaggcggag gcggcggcgg cggcggcgaa gatggacgag
      181 ctggcgggag gaggaggagg aggcggcggc gactggtcgt acctcgcggc ggacgcgctg
      241 gcggcggcgt cgttcacggc gttcccgttc caccaccacc accaccacca ccaccgcgac
      301 gtgctctcgg cgtcgacccc gtcgtcgctg ctcctcaaca tggacgcggc caccgccgcg
      361 gccatgttcg acttccaggc ggccttcccg tcgtcctccg tcccgccgcc gccgcccacg
      421 acgacggcgg cgctcccgcc gttccacgac ttcgcctcct ccaacccgtt cgacgacgcg
      481 ccgcccccgt tcctcgcgcc gccggggcag aagctcgggt tcctggggcc ccccggcggc
      541 gcgttcggcg gcgggatggg gtgggacgac gacgacgaga tcgagcagag cgtggacgcc
      601 tcgtccatgg gtgtctcggc ctcgctggag aatgccgcgc ccgtggcggc cggaggaggc
      661 ggcggcggtg gcggcggcgg tgggaggggc aagaagaa
//
