LOCUS       sequence-342519-        1554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Bar1 gene of Eremothecium cymbalariae (barrier part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK031
FEATURES             Location/Qualifiers
     source          1..1554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctgctag ttattcattc tatgttattc atcacgcttt tgttgttccc aaagttggtt
       61 ttcggtggct tgttacatcc tcaagaatgg gatgatactt ccaatgatgg tgaaaaatgt
      121 ttttccatgg atttaatcaa caataatgac cattatttgg cagaaattga agttggtacc
      181 ccacctcaaa agctgaaggt tctgttcgac accggttctg ccgatttgtg gtttcagtcc
      241 tcggataatg aatattgtgc tgctagttac ccccaaggtg acaattctac caacacgaac
      301 gataacgaac aaaacatggc ttccttttgt tcatcttcga gaacgtacaa ttttcaaaga
      361 tcaagcactt tcactcctac cggctataaa ttttttattg attatgtaaa cacattttct
      421 agaggtatat gggcaaaaga tagattggtc attggtaacg cagatgtttc tggtttgcaa
      481 ttcggcgttt ctaacatctc gaatacgact attaatggta ttttgggtgt cggatatgaa
      541 agattagaat ccgttaaggg ttatagcggc gcacccaaca ggacatatcc aaatctacca
      601 atgctattga aagaatcagg taaaatcgat aaagttgctt attcagtctt tttaaatcat
      661 ctgaactcta atgtctcgac ggtgactttt ggcggcgtcg actcagaaaa gtacaccggc
      721 gacttataca agtttccgtt ggtgaatatt tacaaatcta tcccaagacc atccaagttc
      781 catataactt tacaagcact ggaggttcat ggtaattatt cctccttgaa caaaactgtt
      841 tcaaatactc cattgcctgc tttgtttgat actggaacta acggtatggt tgttccacct
      901 aatgttgccg aaaatatcgc atccacaata catgcgacct ttgatgaaaa gttctctttg
      961 tatctatttg cctgtccagc tgaagaagat gacacaaaat tggtcttcga ttttggtgat
     1021 atccaaatcg aggccccact cgccaacttt atagttcctg cagccgaatt aggaacagaa
     1081 caatgtgggg tcgccattat acctgaggat ggtacattca ccctgggaat accatttttg
     1141 agatccacct acgttgtgta caacttggaa gactacgaag tagcccttgc tcaagctaat
     1201 tggaaatggg acggaaagtc taaaattaca cctatcatta gtaacattcc cggcgctaca
     1261 caagccactg ctacgccatg gaacacgaat atggaacagt cgactgattc aatcagctct
     1321 acgtttggta tcacagatat ttcaccttcg acaatgtacc caacaggcac tcaaacaaca
     1381 accatctctg atcatatcac taaatataca gtagaaccac tgcactctcc taacactttg
     1441 accttcacaa atatatctcc aacactcact tctcaagttt ccaccgtatt cattgttact
     1501 actaccgtta ccgcgaccac tacaattact ttgtctcatt gtcaaaaaag aatt
//
