LOCUS       sequence-34252-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: pLenti-EF1a-Backbone(NN)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..72)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(94..243)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(268..288)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcctcgccc ttgctcactg ggccaggatt ctcctcgacg tcaccgcatg ttagcagact
       61 tcctctgccc tctccactgc ctctagagtt aatcagcatg tccaggtcga aatcatcaag
      121 ggcgtcactg ccgagcatgt cgaggtcaaa gtcatcaagg gcatccgaac caagcatgtc
      181 aaggtcaaaa tcatcgaggg cgtcacttcc cagcatatcc agatcaaaat cgtccaatgc
      241 gtcagcccgt ccggaaccgc tggcctccac ctttctcttc ttcttggggc tagctgacgc
      301 gcgagtttgg tccccttcat gcatgggcga gggcgcgtcc aaatccctct ccagcgagtc
      361 tgcgaacgca tgaaggctcg cctggtcagg tgtctgagtt gacgtaggtg aaggcttcgc
      421 gcgtttcata ccgctcgctt ggagaatcct gtcccaccgc tgggaggcgg gaggcagtgt
      481 gcccgagcgg gcctcgagtt ctgtgacacc gactctacga aagagctgca gcagtccgtg
      541 tctcgacata ccaaattgag tcatggcgtc atcgaacgct tgtgcggggt gggagtgaca
      601 ctggaagaat ccgagcacgc ggaccacttg cgcgtgatct gccactcgat gtgatgtcct
      661 ctcgggaatc cttctgttgg tccgcttaat caatgcaggc gcgtgcggga gccccttctt
      721 caccgcatcc aaggcgggtc gtccgccgag gcaggccagc gctacaagat gatcattagt
      781 gagtgcggcc agcgcggggt ccggcctcga aagctgggcc acgattgact ccagtgcggg
      841 tctgcccccg ttattggatg caatagccac tacctgctc
//
