LOCUS       sequence-342521-        1533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Bar1 gene of Kluyveromyces lactis (barrier part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK032
FEATURES             Location/Qualifiers
     source          1..1533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgattttac cttctctcac aattctatgt ttactgacat ctttaataaa ctgtcgtcaa
       61 atagagttcg atcatgattc aaaagtcatt cagctgcaac tacttaaacc agctgataga
      121 agtctacaga aaagatctaa aagttttgaa agtaaactaa atccacagga ctacttttat
      181 accgttgaac ttggtgtagg tacaccatcg cagaaaataa attgtatatt cgatactgga
      241 tcgtcagatt tatgggtacc ggctaagagt aatccattct gtctaggaaa caagctagat
      301 acaaatggta ctaatgaatt ccatggcaca gaaataacac caatactgga ttgcgataag
      361 attggcgttt tcgacccagg ttcgtcctac agtctgagtg caactgggta taatttctcc
      421 actcagtact ttgacggcag ctattcagat gggttttggg tcgtcgatga tatttcaatt
      481 gccgaccagc aggtcccaga tctgcaattt gcagttgcta atatatcaag tgctccatca
      541 ggtgtactag gtgtgggact tcctagactg gaagcagttc gtggatacga tggagccccc
      601 aatgcgaatt acgataactt tccgcagaca ctgaaaagta atagattgat caatagggtg
      661 ttgtactcgt tgtatttcga tagtggagat ctagaaagtg gaacagtgct cttcggaggt
      721 atcgatgaga aaaaatacga tggtgtgctg tatactttgc cattagtcaa tatatatgaa
      781 cagattgatg aacccgctgc ttttgatatc actttacaag gacttggaat aagaagtgaa
      841 tcgaattgta aagatactgt catcgcgcat aaaaaggccc cagcactact tgattctggt
      901 agtacaatca tgggagctcc tcaagaggca ctagaaagta ttgcccaaga actgggcgct
      961 gcattctcgg aaagcgacgg gttgtatgtg ttagattgcc cagcatccaa tgatgatcgc
     1021 acattttatt tcgatttcgg tgaattgaaa ctgaaagtgc caatacatga cctcttatat
     1081 ctacctgaat ccggtcagaa ttattgtggg ttggcaataa ttgcgagtgg cgaagagtgg
     1141 atcctggggg atcttgtttt gaaaagcgca tatgtagtat atgaccttga taacttggag
     1201 atctctattt cccaggtaaa aaccacgagc gggtcaagaa tacaaacagt aagtgcaaca
     1261 ggctctctgc ctcaaactag aaaatcaaat tctaccccct ggagtctgtc tggcagtacc
     1321 aactcaagta cagactctgt atttgataag cctttaccta agaaatgtca aaaatcaccc
     1381 tctaagacat gtccggcaaa gaaaaaagga aataaaagag aagtcttaat caatatacat
     1441 gacaatctaa ttatgcttga aaactctggt tcttctagtt taagaccatc ctatctgctt
     1501 tgcgcattgt tagtactctt ccattctctc cta
//
