LOCUS       sequence-342532-        8632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses DCAF15 in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Faust TB, Yoon H, Nowak RP, Donovan KA, Li Z, Cai Q, Eleuteri NA, 
            Zhang T, Gray NS, Fischer ES
  TITLE     Structural complementarity facilitates E7820-mediated degradation of
            RBM39 by DCAF15.
  JOURNAL   Nat Chem Biol. 2020 Jan;16(1):7-14. doi: 10.1038/s41589-019-0378-3. 
            Epub 2019 Nov 4.
  PUBMED    31686031
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0378-3"; journalName: "Nat Chem Biol"; date: 
            "2020-01"; volume: "16"; issue: "1"; pages: "7-14"
COMMENT     Sequence Label: pHY002_Strep_Avi_hsDCAF15
FEATURES             Location/Qualifiers
     source          1..8632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      89..677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     578..597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     831..848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_recomb     1240..2067
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             1240..1364
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(1402..2007)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     protein_bind    complement(2071..2104)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2294..2342
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2367..2386
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(2420..3097)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     complement(2680..2699)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(3106..3215)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        3270..3361
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3286..3303
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3379..3402
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3412..3456
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             3475..3495
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_recomb     5344..6701
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(5360..6701)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      6848..7303
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6935..6954)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7145..7166
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7320..7339)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7439..7461
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7499..7517)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7585..7689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7690..7758,7759..8550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7908..7927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
       61 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
      781 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      841 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      901 ttcattaatg cagaacggct ccgcccacta ttaatgaaat taaaaattcc aattttaaaa
      961 aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa aatgtcgtcg
     1021 acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg aacgatttga
     1081 aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta aactgttaca
     1141 ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag gctctgacgc
     1201 atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta atcaaatccc
     1261 aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag ctctgtccgt
     1321 ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata aaacaattat
     1381 aaatgtcaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa catttgtagt
     1441 attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa aatcattttc
     1501 aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac tagacgcctt
     1561 gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa aattaacata
     1621 gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt tgtcataaat
     1681 atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc tgtaatttac
     1741 aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta aatttatata
     1801 atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt acgcagcttc
     1861 ttctagttca attacaccat tttttagcag caccggatta acataacttt ccaaaatgtt
     1921 gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt ctgcgagcag
     1981 ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat atcacaaact
     2041 ggaaatgtct atcaatatat agttgctgat ataacttcgt atagcataca ttatacgaag
     2101 ttatctggtt gaaacgtacc cgtagtggct atggcagggc ttgccgcccc gacgttggct
     2161 gcgagccctg ggccttcacc cgaacttggg ggttggggtg gggaaaagga agaaacgcgg
     2221 gcgtattggt cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga
     2281 accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc
     2341 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca
     2401 tctcccgcta ttactcgagt cactgggagc cggagtggcg ggcctcggcg tgctcgtact
     2461 gctccaccac ggtgtagtcc tcgttgtggt tggtgatgtc cagcttggag tccacgtagt
     2521 ggttgccggg cagctgcacg ggcttcttgg ccttgtacac ggtcttgaag tcgcaggtgt
     2581 agtggccgcc gtccttcagc ttcagggcgt gggagatctc gcccttcagc acgccgtcct
     2641 gggggtacag cttctcggtg gagggctccc agccggcagt cttcttctgc attacggggc
     2701 cgtcggcggg gaagttcacg cccttgaact tcaccttgta gatgaaggtg ccgtcctgca
     2761 gggaggagtc ctgctgcacc tccaccacgc cgccgtcctc gaagttcatg gagcgctccc
     2821 aggtgaagcc ctcggggaag gacagcttca tgtagtcggg gatgtcggcg gggtgcttca
     2881 cgtaggcctt ggagccgtac tggaactggg gggacaggat gtcccaggcg aagggcaggg
     2941 ggccgccctt ggtcacctgc agcttggcgg tctgggtgcc ctcgtagggc ttgccctcgc
     3001 cctcgccctc gatctcgaag tagtggccgt tcacggagcc ctccatgcgc accttgaact
     3061 gcatgaactc cttgatgacg tcctcggtgt tgtccatggt agagtgattg taaataaaat
     3121 gtaatttaca gtatagtatt ttaattaata tacaaatgat ttgataataa ttcttattta
     3181 actataatat attgtgttgg gttgaattaa aggtccgtat actagtatcg attcgcgacc
     3241 tactccggaa tattaataga gttgctgata tcatggagat aattaaaatg ataaccatct
     3301 cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa aacctataaa
     3361 tacggatcta ccatggactg gagccacccg cagttcgaaa aatccgcggt cggcctgaac
     3421 gacatcttcg aggctcagaa aatcgaatgg cacgaaggtg gaggaggttc tggagagaac
     3481 ctctacttcc agggaggcgg ccgcatggcg ccgagttcta aatcggagcg aaattcgggc
     3541 gctggttctg gtggcggggg tcccggggga gctgggggca aacgtgcagc aggtcggcgc
     3601 agagagcatg ttctgaaaca gctcgagagg gttaagatct ccgggcaact gtcgcccagg
     3661 cttttccgta agctccctcc acgagtttgt gtttccctca agaatattgt tgacgaggat
     3721 tttttgtatg ctggtcatat tttcctcggg ttctcgaaat gtggccgata cgtgttgtcg
     3781 tatacttcta gctctggaga tgatgatttc tctttctaca tctaccactt gtattggtgg
     3841 gaatttaacg ttcacagcaa actgaaactg gtgagacaag ttcgtttgtt ccaggatgag
     3901 gaaatttata gtgatctcta ccttacagtt tgtgagtggc cctccgacgc ttcgaaggtt
     3961 attgtgtttg gatttaatac ccgaagcgca aacggtatgt tgatgaatat gatgatgatg
     4021 tcggatgaaa accatcgcga tatctacgtg tccacggttg ctgtgcctcc accgggacga
     4081 tgtgctgcct gtcaagacgc gtctcgagct caccccggcg atcctaatgc acaatgtctg
     4141 aggcacgggt ttatgcttca tacgaaatat caagtcgttt atcccttccc tacgtttcag
     4201 ccagcatttc agctgaagaa ggatcaagtt gttctcctca acacctcgta cagccttgtt
     4261 gcctgtgcgg tgtctgtcca ttctgccggc gaccggtctt tctgccagat cctgtacgat
     4321 catagtacat gcccgttggc tcccgcatcg cctccagagc cacaaagtcc tgagctgcct
     4381 ccggctctgc cttcgttctg cccagaagcg gctcccgcgc gcagtagcgg aagtcctgag
     4441 ccttcgcccg ctattgcaaa agccaaggag tttgttgctg acatttttcg gcgagctaaa
     4501 gaagctaagg gaggtgtgcc cgaagaagcc cgtcctgcgc tttgtccagg acctagcggt
     4561 tcccgttgcc gcgcccattc cgagcctctc gctctttgcg gagaaaccgc gccgagagac
     4621 agtccccccg cgagtgaggc tccagcgtcc gagccgggtt acgtcaatta tacgaagttg
     4681 tattatgtgt tggaatccgg agaaggcaca gaaccggagg acgaactcga ggacgacaaa
     4741 atttcccttc cctttgttgt cacagacctc cgggggcgca atctgcgacc aatgcgggag
     4801 cgcacggctg tgcaaggaca ataccttacc gttgagcaac tgactctcga ttttgaatat
     4861 gtgattaatg aggtcattcg ccacgacgcc acgtgggggc accaattttg tagcttttcg
     4921 gattatgata ttgtcatcct cgaagtttgc cccgagacga atcaagtctt gattaacatc
     4981 ggcttgctct tgctcgcatt tccctccccg accgaggagg gtcaactgag gcccaaaaca
     5041 tatcacacat ctttgaaggt tgcttgggac ctcaacacgg gtatcttcga aaccgttagc
     5101 gttggagact tgacagaggt taaagggcag accagtgggt ccgtgtggag ttcttatcgc
     5161 aaaagctgcg tcgacatggt catgaagtgg ttggttccgg agagttcggg acgctacgtc
     5221 aacagaatga cgaacgaggc gctgcacaag ggttgtagct tgaaagtcct cgctgattcg
     5281 gagcgataca cctggattgt tttgtgaggt acccccggac ggccgcttaa ttaattgatc
     5341 cgggttatta gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt
     5401 gttgtacgta ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga
     5461 aaatcaaatg attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt
     5521 tgtaaaatag gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac
     5581 tatctaatgg attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag
     5641 ctttgtcgat attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat
     5701 tatgcgcttt tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca
     5761 cgttaaataa agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat
     5821 cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca
     5881 cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt
     5941 ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt
     6001 ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca
     6061 aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag
     6121 gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag
     6181 gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg
     6241 ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca
     6301 acaattgttg tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc attggtggag
     6361 cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg aggcgctgga atgttaggca
     6421 cgggagaagg tggtggcggc ggtgccgccg gtataatttg ttctggttta gtttgttcgc
     6481 gcacgattgt gggcaccggc gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc
     6541 gaggcagcgc ttggggtggt ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat
     6601 ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa
     6661 tgtaagcaat tgtattgtaa agagattgtc tcaagctcgg atcgatcccg cacgccgata
     6721 acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct gtcgtcgcct
     6781 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     6841 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6901 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6961 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7021 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     7081 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7141 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     7201 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7261 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     7321 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     7381 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7441 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     7501 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     7561 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7621 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7681 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7741 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7801 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7861 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7921 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7981 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8041 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8101 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8161 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8221 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8281 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8341 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8401 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8461 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8521 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8581 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cc
//
