LOCUS       sequence-342534-        5381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized 
            for S. typhimurium).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Lane K, Andres-Terre M, Kudo T, Monack DM, Covert MW
  TITLE     Escalating Threat Levels of Bacterial Infection Can Be Discriminated
            by Distinct MAPK and NF-kappaB Signaling Dynamics in Single Host 
            Cells.
  JOURNAL   Cell Syst. 2019 Mar 27;8(3):183-196.e4. doi: 
            10.1016/j.cels.2019.02.008. Epub 2019 Mar 20.
  PUBMED    30904375
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2019.02.008"; journalName: "Cell Syst"; date: 
            "2019-03-27- 27"; volume: "8"; issue: "3"; pages: "183-196.e4"
COMMENT     Sequence Label: pFPV25.1_rpsM_mCerulean3
FEATURES             Location/Qualifiers
     source          1..5381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1793..1811
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            3811..3898
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     primer_bind     complement(5211..5228)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgga tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1801 tatcacgagg ccctttcgtc ttcaagaatt cgagctcggt acccggggat cccggagttc
     1861 atgcgtgatg caatgaaagt gccgttctac ttcggtggga cctcactgct tatcgttgtt
     1921 gtcgtgatta tggactttat ggctcaagtg caaactctga tgatgtccag tcagtatgag
     1981 tctgcattga agaaggcgaa cctgaaaggc tacggccgtt aattggtcgc ctgagaagtt
     2041 acggagagta aaaatgaaag ttcgtgcttc cgtcaagaaa ttatgccgta actgcaaaat
     2101 cgttaagcgt gatggtgtca tccgtgtgat ttgcagtgcc gagccgaagc ataaacagcg
     2161 ccaaggctga ttttttcgca tatttttctt gcaaagttgg gttgagctgg ctagattagc
     2221 cagccaatct tttgtatgtc tgtacgtttc catttgagta tcctgaaaac gggcttttca
     2281 gcatggtacg tacatattaa atagtaggag tgcatagtgg cccgtatagc aggcattaac
     2341 attcctgatc agaaacacgc cgtgatcgcg ttaacttcga tctacggtgt cggcaagacc
     2401 cgttctaaag ccatcctggc tgcagcgggt atcgctgaaa atgttaagat cctctagatt
     2461 taagaaggag atatacatat ggtatcaaaa ggtgaagaat tgtttactgg agttgtacct
     2521 attttggttg agctggatgg ggatgtaaat ggacataagt tctccgtgtc tggggaaggc
     2581 gagggggatg cgacatatgg taagcttaca ttaaaattca tttgtactac gggcaagtta
     2641 cctgtgccgt ggccgacctt ggtgacgaca ctttcctggg gagttcaatg cttcgctcgc
     2701 tacccggacc acatgaagca gcacgatttc ttcaagtctg ctatgcccga aggttacgtt
     2761 caggaacgca ccatcttttt caaggatgat ggtaattata agactcgcgc tgaagtcaaa
     2821 ttcgagggcg acaccttagt caatcgcatt gaacttaagg gcatcgactt taaggaagat
     2881 ggaaatatcc tgggccacaa actggaatac aatgccattc acggaaatgt gtatatcact
     2941 gctgataagc agaagaatgg cattaaagcg aactttggcc ttaattgcaa catcgaggat
     3001 ggctcggtac aattagcaga ccattatcaa caaaatactc ctatcgggga tggtcctgtt
     3061 cttctgccag acaatcacta tttatcaaca cagagcaagt tgtctaaaga tccaaatgag
     3121 aagcgggatc atatggtgtt acttgaattc gtgacagccg cgggtattac tcttggcatg
     3181 gacgaattat ataagtaagc atgcaagctt aatgcggtag tttatcacag ttaaattgct
     3241 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     3301 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     3361 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg cttttagccg
     3421 ctttagcggc ctttccccct acccgaaggg tgggggcgcg tgtgcagccc cgcagggcct
     3481 gtctcggtcg atcattcagc ccggctcatc cttctggcgt ggcggcagac cgaacaaggc
     3541 gcggtcgtgg tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat cctccggcca
     3601 ctcgctgctg ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc gccttgtttc
     3661 gttcttgcgc tcttgctgct gttcccttgc ccgcacccgc tgaatttcgg cattgattcg
     3721 cgctcgttgt tcttcgagct tggccagccg atccgccgcc ttgttgctcc ccttaaccat
     3781 cttgacaccc cattgttaat gtgctgtctc gtaggctatc atggaggcac agcggcggca
     3841 atcccgaccc tactttgtag gggagggcgc acttaccggt ttctcttcga gaaactggcc
     3901 taacggccac ccttcgggcg gtgcgctctc cgagggccat tgcatggagc cgaaaagcaa
     3961 aagcaacagc gaggcagcat ggcgatttat caccttacgg cgaaaaccgg cagcaggtcg
     4021 ggcggccaat cggccagggc caaggccgac tacatccagc gcgaaggcaa gtatgcccgc
     4081 gacatggatg aagtcttgca cgccgaatcc gggcacatgc cggagttcgt cgagcggccc
     4141 gccgactact gggatgctgc cgacctgtat gaacgcgcca atgggcggct gttcaaggag
     4201 gtcgaatttg ccctgccggt cgagctgacc ctcgaccagc agaaggcgct ggcgtccgag
     4261 ttcgcccagc acctgaccgg tgccgagcgc ctgccgtata cgctggccat ccatgccggt
     4321 ggcggcgaga acccgcactg ccacctgatg atctccgagc ggatcaatga cggcatcgag
     4381 cggcccgccg ctcagtggtt caagcggtac aacggcaaga ccccggagaa gggcggggca
     4441 cagaagaccg aagcgctcaa gcccaaggca tggcttgagc agacccgcga ggcatgggcc
     4501 gaccatgcca accgggcatt agagcgggct ggccacgacg cccgcattga ccacagaaca
     4561 cttgaggcgc agggcatcga gcgcctgccc ggtgttcacc tggggccgaa cgtggtggag
     4621 atggaaggcc ggggcatccg caccgaccgg gcagacgtgg ccctgaacat cgacaccgcc
     4681 aacgcccaga tcatcgactt acaggaatac cgggaggcaa tagaccatga acgcaatcga
     4741 cagagtgaag aaatccagag gcatcaacga gttagcggag cagatcgaac cgctggccca
     4801 gagcatggcg acactggccg acgaagcccg gcaggtcatg agccagaccc agcaggccag
     4861 cgaggcgcag gcggcggagt ggctgaaagc ccagcgccag acaggggcgg catgggtgga
     4921 gctggccaaa gagttgcggg aggtagccgc cgaggtgagc agcgccgcgc agagcgcccg
     4981 gagcgcgtcg cgggggtggc actggaagct atggctaacc gtgatgctgg cttccatgat
     5041 gcctacggtg gtgctgctga tcgcatcgtt gctcttgctc gacctgacgc cactgacaac
     5101 cgaggacggc tcgatctggc tgcgcttggt ggcccgatga agaacgacag gactttgcag
     5161 gccataggcc gacagctcaa ggccatgggc tgtgagcgct cttccgcttc ctcgctcact
     5221 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5281 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5341 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt t
//
