LOCUS       sequence-34254-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: pLenti-EF1a-Backbone(NI)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..73)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(95..244)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(269..289)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctcctcgcc cttgctcact gggccaggat tctcctcgac gtcaccgcat gttagcagac
       61 ttcctctgcc ctctccactg cctctagagt taatcagcat gtccaggtcg aaatcatcaa
      121 gggcgtcact gccgagcatg tcgaggtcaa agtcatcaag ggcatccgaa ccaagcatgt
      181 caaggtcaaa atcatcgagg gcgtcacttc ccagcatatc cagatcaaaa tcgtccaatg
      241 cgtcagcccg tccggaaccg ctggcctcca cctttctctt cttcttgggg ctagctgacg
      301 cgcgagtttg gtccccttca tgcatgggcg agggcgcgtc caaatccctc tccagcgagt
      361 ctgcgaacgc atgaaggctc gcctggtcag gtgtctgagt tgacgtaggt gaaggcttcg
      421 cgcgtttcat accgctcgct tggagaatcc tgtcccaccg ctgggaggcg ggaggcagtg
      481 tgcccgagcg ggcctcgagt tctgtgacac cgactctacg aaagagctgc agcagtccgt
      541 gtctcgacat accaaattga gtcatggcgt catcgaacgc ttgtgcgggg tgggagtgac
      601 actggaagaa tccgagcacg cggaccactt gcgcgtgatc tgccactcga tgtgatgtcc
      661 tctcgggaat ccttctgttg gtccgcttaa tcaatgcagg cgcgtgcggg agccccttct
      721 tcaccgcatc caaggcgggt cgtccgccga ggcaggccag cgctacaaga tgatcattag
      781 tgagtgcggc cagcgcgggg tccggcctcg aaagctgggc cacgattgac tccagtgcgg
      841 gtctgccccc gatattggat gcaatagcca ctacctgctc
//
