LOCUS       sequence-342548-       12370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of Cypridina luciferase, puromycin 
            N-acetyl-transferase and shRNAmir of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   Charles JP, Fuchs J, Hefter M, Vischedyk JB, Kleint M, Vogiatzi F, 
            Schafer JA, Nist A, Timofeev O, Wanzel M, Stiewe T
  TITLE     Monitoring the dynamics of clonal tumour evolution in vivo using 
            secreted luciferases.
  JOURNAL   Nat Commun. 2014 Jun 3;5:3981. doi: 10.1038/ncomms4981.
  PUBMED    24889111
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-3"; volume: "5:3981. doi"; pages: " 
            10.1038/ncomms4981"
COMMENT     Sequence Label: pCLucIPZ
FEATURES             Location/Qualifiers
     source          1..12370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    669..902
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1087..1131
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1429..1546
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1611..1985)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1982..1991
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1995..2013)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2005..2052)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        2157..2460
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2461..2664
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2615..2635
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2736..4397
                     /codon_start=1
                     /product="secreted Cypridina luciferase"
                     /label=CLuc
                     /note="contains 30 codon substitutions for efficient 
                     translation in mammalian cells"
                     /translation="MKTLILAVALVYCATVHCQDCPYEPDPPNTVPTSCEAKEGECIDS
                     SCGTCTRDILSDGLCENKPGKTCCRMCQYVIECRVEAAGWFRTFYGKRFQFQEPGTYVL
                     GQGTKGGDWKVSITLENLDGTKGAVLTKTRLEVAGDIIDIAQATENPITVNGGADPIIA
                     NPYTIGEVTIAVVEMPGFNITVIEFFKLIVIDILGGRSVRIAPDTANKGMISGLCGDLK
                     MMEDTDFTSDPEQLAIQPKINQEFDGCPLYGNPDDVAYCKGLLEPYKDSCRNPINFYYY
                     TISCAFARCMGGDERASHVLLDYRETCAAPETRGTCVLSGHTFYDTFDKARYQFQGPCK
                     EILMAADCFWNTWDVKVSHRNVDSYTEVEKVRIRKQSTVVELIVDGKQILVGGEAVSVP
                     YSSQNTSIYWQDGDILTTAILPEALVVKFNFKQLLVVHIRDPFDGKTCGICGNYNQDFS
                     DDSFDAEGACDLTPNPPGCTEEQKPEAERLCNSLFAGQSDLDQKCNVCHKPDRVERCMY
                     EYCLRGQQGFCDHAWEFKKECYIKHGDTLEVPDECK"
     misc_feature    4414..4986
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4581..4598)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4808..4827
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4989..5591
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5488..5508
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     ncRNA           5598..5719
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           5747..5761
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           5789..5915
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(6010..6028)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6048..6636
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6101..6121)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6519..6530)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6843..7076
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7177..7194)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7183..7407
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7453..7881
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7540..7559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7750..7771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7890..7910)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7895..8224
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8075..8210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8137..8156
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9091..9212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9261..9277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9261..9277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9274..9296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9285..9301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9309..9315,9316..9333,9334..9339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9354..9375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9492..9509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9663..10251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9743..9762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10422..11213,11214..11282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11045..11064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11283..11387)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11435..11569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             11737..12370
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
       61 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      121 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      181 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      241 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      301 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      361 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      421 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      481 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
      541 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
      601 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
      661 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
      721 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
      781 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
      841 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
      901 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
      961 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1021 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1081 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1141 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1201 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     1261 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     1321 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     1381 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     1441 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1501 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     1561 ggacagcaga gatccagttt ggttagtacc gggcccgctc tagtccggaa tcagtcctgc
     1621 tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc
     1681 cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac
     1741 acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg
     1801 gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg
     1861 accacaccgg cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag
     1921 aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg
     1981 gccatggtgg ccctcctata gtgagtcgta ttatactatg ccgatatact atgccgatga
     2041 ttaattgtca acacgtgctg caggtccgag gttctagacg tattaccgcc atgcattagt
     2101 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     2161 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     2221 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2281 gtggagtatt tacggtaaac tacccacttg gcagtacatc aagtgtatca tatgccaagt
     2341 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2401 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2461 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2521 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2581 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2641 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
     2701 gcctgcaggt tggtaccgag ctcggatccg ccaccatgaa gaccttaatt cttgccgttg
     2761 cattagtcta ctgcgccact gttcattgcc aggactgtcc ttacgaacct gatccaccaa
     2821 acacagttcc aacttcctgt gaagctaaag aaggagaatg tattgatagc agctgtggca
     2881 cctgcacgag agacatacta tcagatggac tgtgtgaaaa taaaccagga aaaacatgtt
     2941 gccgaatgtg tcagtatgta attgaatgca gagtagaggc cgcaggatgg tttagaacat
     3001 tctatggaaa gagattccag ttccaggaac ctggtacata cgtgttgggt caaggaacca
     3061 agggcggcga ctggaaggtg tccatcaccc tggagaacct ggatggaacc aagggggctg
     3121 tgctgaccaa gacaagactg gaagtggctg gagacatcat tgacatcgct caagctactg
     3181 agaatcccat cactgtaaac ggtggagctg accctatcat cgccaacccg tacaccatcg
     3241 gcgaggtcac catcgctgtt gttgagatgc caggcttcaa catcaccgtc attgagttct
     3301 tcaaactgat cgtgatcgac atcctcggag gaagatctgt aagaatcgcc ccagacacag
     3361 caaacaaagg aatgatctct ggcctctgtg gagatcttaa aatgatggaa gatacagact
     3421 tcacttcaga tccagaacaa ctcgctattc agcctaagat caaccaggag tttgacggtt
     3481 gtccactcta tggaaatcct gatgacgttg catactgcaa aggtcttctg gagccgtaca
     3541 aggacagctg ccgcaacccc atcaacttct actactacac catctcctgc gccttcgccc
     3601 gctgtatggg tggagacgag cgagcctcac acgtgctgct tgactacagg gagacgtgcg
     3661 ctgctcccga aactagagga acctgcgttt tgtctggaca tactttctac gatacatttg
     3721 acaaagcaag ataccaattc cagggtccct gcaaggagat tcttatggcc gccgactgtt
     3781 tctggaacac ttgggatgtg aaggtttcac acaggaatgt tgactcttac actgaagtag
     3841 agaaagtacg aatcaggaaa caatcgactg tagtagaact cattgttgat ggaaaacaga
     3901 ttctggttgg aggagaagcc gtgtccgtcc cgtacagctc tcagaacact tccatctact
     3961 ggcaagatgg tgacatactg actacagcca tcctacctga agctctggtg gtcaagttca
     4021 acttcaagca actgctcgtc gtacatatta gagatccatt cgatggtaag acttgcggta
     4081 tttgcggtaa ctacaaccag gatttcagtg atgattcttt tgatgctgaa ggagcctgtg
     4141 atctgacccc caacccaccg ggatgcaccg aagaacagaa acctgaagct gaacgactct
     4201 gcaatagtct cttcgccggt caaagtgatc ttgatcagaa atgtaacgtg tgccacaagc
     4261 ctgaccgtgt cgaacgatgc atgtacgagt attgcctgag gggacaacag ggtttctgtg
     4321 accacgcatg ggagttcaag aaagaatgct acataaagca tggagacacc ctagaagtac
     4381 cagatgaatg caaataggcg gccgcaaatt ccgcccctct ccctcccccc cccctaacgt
     4441 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     4501 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     4561 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     4621 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4681 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4741 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4801 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4861 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     4921 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     4981 ataataccat ggccaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5041 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5101 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5161 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5221 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggctcg cgcatggccg
     5281 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     5341 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     5401 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gctggggtgc
     5461 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     5521 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     5581 ccggtgcctg agtttgtttg aatgaggctt cagtacttta cagaatcgtt gcctgcacat
     5641 cttggaaaca cttgctggga ttacttcttc aggttaaccc aacagaaggc tcgagaaggt
     5701 atattgctgt tgacagtgag cgatctcgct tgggcgagag taagtagtga agccacagat
     5761 gtacttactc tcgcccaagc gagagtgcct actgcctcgg aattcaaggg gctactttag
     5821 gagcaattat cttgtttact aaaactgaat accttgctat ctctttgata catttttaca
     5881 aagctgaatt aaaatggtat aaattaaatc acttttttca attggaagac taatgcggcc
     5941 ggccattact ccgtctcgtg tcttgttgca tatgtctgct ggtttgtttg atgttgtttg
     6001 cgggcgggcc ctatagtgag tcgtattacc taggacgcgt ctggaacaat caacctctgg
     6061 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6121 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6181 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6241 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6301 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6361 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6421 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     6481 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6541 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6601 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga
     6661 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     6721 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     6781 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg
     6841 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6901 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6961 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7021 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7081 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7141 tgagaggcct tgacattgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     7201 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7261 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7321 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7381 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     7441 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7501 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7561 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7621 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7681 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7741 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7801 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7861 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7981 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8041 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8161 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8221 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgatgaaaaa
     8281 gcctgaactc accgcgacgt cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     8341 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     8401 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     8461 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     8521 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     8581 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     8641 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     8701 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     8761 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     8821 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     8881 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     8941 cgtccgaggg caaaggaata gcacgtgcta cgagatttcg attccaccgc cgccttctat
     9001 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     9061 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     9121 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9181 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     9241 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9301 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9361 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9421 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9841 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9901 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9961 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    10021 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    10081 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    10141 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    10201 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10261 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10321 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10381 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10441 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10501 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10561 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10621 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10681 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10741 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10801 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10861 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10921 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10981 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    11041 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11101 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11161 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    11221 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11281 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    11341 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11401 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
    11461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    11521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    11581 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    11641 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
    11701 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
    11761 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
    11821 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
    11881 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
    11941 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
    12001 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
    12061 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
    12121 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
    12181 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    12241 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    12301 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    12361 atctctagca
//
