LOCUS       sequence-342553-       15057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PhiC31 integration plasmid for tetO array with GFP expression 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15057)
  AUTHORS   Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, 
            Lomvardas S, Weiner OD
  TITLE     Live-cell imaging reveals enhancer-dependent Sox2 transcription in 
            the absence of enhancer proximity.
  JOURNAL   Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769.
  PUBMED    31124784
REFERENCE   2  (bases 1 to 15057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-05-24"; volume: "8. pii: 41769. doi"; pages: " 
            10.7554/eLife.41769"
COMMENT     Sequence Label: pDEST-tetOx224_PhiC31attB_FRT-EF1aGFP-FRT
FEATURES             Location/Qualifiers
     source          1..15057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..88
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(166..184,185..207,208..226,227..249,250..268,269..291,
                     292..310,311..333,334..352,353..375,376..394,395..417,
                     418..436)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(513..531,532..554,555..573,574..596,597..615,616..638,
                     639..657,658..680,681..699,700..722,723..741,742..764,
                     765..783)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(860..878,879..901,902..920,921..943,944..962,963..985,
                     986..1004,1005..1027,1028..1046,1047..1069,1070..1088,
                     1089..1111,1112..1130)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1207..1225,1226..1248,1249..1267,1268..1290,1291..1309,
                     1310..1332,1333..1351,1352..1374,1375..1393,1394..1416,
                     1417..1435,1436..1458,1459..1477)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1554..1572,1573..1595,1596..1614,1615..1637,1638..1656,
                     1657..1679,1680..1698,1699..1721,1722..1740,1741..1763,
                     1764..1782,1783..1805,1806..1824)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1901..1919,1920..1942,1943..1961,1962..1984,1985..2003,
                     2004..2026,2027..2045,2046..2068,2069..2087,2088..2110,
                     2111..2129,2130..2152,2153..2171)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2248..2266,2267..2289,2290..2308,2309..2331,2332..2350,
                     2351..2373,2374..2392,2393..2415,2416..2434,2435..2457,
                     2458..2476,2477..2499,2500..2518)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2595..2613,2614..2636,2637..2655,2656..2678,2679..2697,
                     2698..2720,2721..2739,2740..2762,2763..2781,2782..2804,
                     2805..2823,2824..2846,2847..2865)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2942..2960,2961..2983,2984..3002,3003..3025,3026..3044,
                     3045..3067,3068..3086,3087..3109,3110..3128,3129..3151,
                     3152..3170,3171..3193,3194..3212)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3289..3307,3308..3330,3331..3349,3350..3372,3373..3391,
                     3392..3414,3415..3433,3434..3456,3457..3475,3476..3498,
                     3499..3517,3518..3540,3541..3559)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3636..3654,3655..3677,3678..3696,3697..3719,3720..3738,
                     3739..3761,3762..3780,3781..3803,3804..3822,3823..3845,
                     3846..3864,3865..3887,3888..3906)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3983..4001,4002..4024,4025..4043,4044..4066,4067..4085,
                     4086..4108,4109..4127,4128..4150,4151..4169,4170..4192,
                     4193..4211,4212..4234,4235..4253)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4330..4348,4349..4371,4372..4390,4391..4413,4414..4432,
                     4433..4455,4456..4474,4475..4497,4498..4516,4517..4539,
                     4540..4558,4559..4581,4582..4600)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4677..4695,4696..4718,4719..4737,4738..4760,4761..4779,
                     4780..4802,4803..4821,4822..4844,4845..4863,4864..4886,
                     4887..4905,4906..4928,4929..4947)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5024..5042,5043..5065,5066..5084,5085..5107,5108..5126,
                     5127..5149,5150..5168,5169..5191,5192..5210,5211..5233,
                     5234..5252,5253..5275,5276..5294)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5371..5389,5390..5412,5413..5431,5432..5454,5455..5473,
                     5474..5496,5497..5515,5516..5538,5539..5557,5558..5580,
                     5581..5599,5600..5622,5623..5641)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5718..5736,5737..5759,5760..5778,5779..5801,5802..5820,
                     5821..5843,5844..5862,5863..5885,5886..5904,5905..5927,
                     5928..5946,5947..5969,5970..5988)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6065..6083,6084..6106,6107..6125,6126..6148,6149..6167,
                     6168..6190,6191..6209,6210..6232,6233..6251,6252..6274,
                     6275..6293,6294..6316,6317..6335)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6412..6430,6431..6453,6454..6472,6473..6495,6496..6514,
                     6515..6537,6538..6556,6557..6579,6580..6598,6599..6621,
                     6622..6640,6641..6663,6664..6682)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6759..6777,6778..6800,6801..6819,6820..6842,6843..6861,
                     6862..6884,6885..6903,6904..6926,6927..6945,6946..6968,
                     6969..6987,6988..7010,7011..7029)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7106..7124,7125..7147,7148..7166,7167..7189,7190..7208,
                     7209..7231,7232..7250,7251..7273,7274..7292,7293..7315,
                     7316..7334,7335..7357,7358..7376)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7453..7471,7472..7494,7495..7513,7514..7536,7537..7555,
                     7556..7578,7579..7597,7598..7620,7621..7639,7640..7662,
                     7663..7681,7682..7704,7705..7723)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7800..7818,7819..7841,7842..7860,7861..7883,7884..7902,
                     7903..7925,7926..7944,7945..7967,7968..7986,7987..8009,
                     8010..8028,8029..8051,8052..8070)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8147..8165,8166..8188,8189..8207,8208..8230,8231..8249,
                     8250..8272,8273..8291,8292..8314,8315..8333,8334..8356,
                     8357..8375,8376..8398,8399..8417)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8494..8512,8513..8535,8536..8554,8555..8577,8578..8596,
                     8597..8619,8620..8638,8639..8661,8662..8680,8681..8703,
                     8704..8722,8723..8745,8746..8764)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8841..8859,8860..8882,8883..8901,8902..8924,8925..8943,
                     8944..8966,8967..8985,8986..9008,9009..9027,9028..9050,
                     9051..9069,9070..9092,9093..9111)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(9144..9160)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(9160..9184)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     9208..9224
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9261..9277)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9495..9564
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(10061..10078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(10176..10209)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      complement(10270..10858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10350..10369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11029..11820,11821..11889))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11652..11671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11890..11994)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12021..12476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12108..12127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12318..12339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12602..12624
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12616..12633
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12617..12633
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    12703..12927
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     12916..12933
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(12963..13676,13677..13679,13680..13682))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(12966..13676,13677..13679,13680..13682))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(13008..13029)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     13357..13376
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     13616..13637
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      13679..13688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(13695..14873)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          13704..14642
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(13727..13747)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(14990..15023)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    join(15057,1..24)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc
       61 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
      121 ggatccgtat cacgaggccc tttcgtcttc actcgagttt accactccct atcagtgata
      181 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      241 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      301 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      361 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      421 ctatcagtga tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac
      481 gaggcccttt cgtcttcact cgagtttacc actccctatc agtgatagag aaaagtgaaa
      541 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      601 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      661 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      721 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      781 agaaaagtga aagtcgggct cssttcccgs gtcgagatcc gtatcacgag gccctttcgt
      841 cttcactcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
      901 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      961 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     1021 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     1081 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     1141 tcgggctcgg ttcccgggtc gagatccgta tcacgaggcc ctttcgtctt cactcgagtt
     1201 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     1261 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     1321 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     1381 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     1441 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc
     1501 ccgggtcgag atccgtatca cgaggccctt tcgtcttcac tcgagtttac cactccctat
     1561 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     1621 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     1681 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     1741 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     1801 accactccct atcagtgata gagaaaagtg aaagtcgggc tcggttcccg ggtcgagatc
     1861 cgtatcacga ggccctttcg tcttcactcg agtttaccac tccctatcag tgatagagaa
     1921 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgrgttta
     1981 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     2041 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2101 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2161 agtgatagag aaaagtgaaa gtcgggctcg gttcccgggt cgagatccgt atcacgaggc
     2221 cctttcgtct tcactcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2281 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2341 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2401 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2461 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2521 agtgaaagtc gggctcggtt cccgggtcga gatccgtatc acgaggccct ttcgtcttca
     2581 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2641 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2701 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2761 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2821 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcggg
     2881 ctcggttccc gggtcgagat ccgtatcacg aggccctttc gtcttcactc gagtttacca
     2941 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     3001 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     3061 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     3121 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     3181 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgggctc ggttcccggg
     3241 tcgagatccg tatcacgagg ccctttcgtc ttcactcgag tttaccactc cctatcagtg
     3301 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     3361 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     3421 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     3481 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     3541 tccctatcag tgatagagaa aagtgaaagt cgggctcggt tcccgggtcg agatccgtat
     3601 cacgaggccc tttcgtcttc actcgagttt accactccct atcagtgata gagaaaagtg
     3661 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     3721 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     3781 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     3841 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     3901 tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac gaggcccttt
     3961 cgtcttcact cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     4021 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     4081 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     4141 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     4201 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     4261 aagtcgggct cggttcccgg gtcgagatcc gtatcacgag gccctttcgt cttcactcga
     4321 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     4381 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     4441 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     4501 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     4561 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgggctcgg
     4621 ttcccgggtc gagatccgta tcacgaggcc ctttcgtctt cactcgagtt taccactccc
     4681 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     4741 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     4801 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     4861 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     4921 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc ccgggtcgag
     4981 atccgtatca cgaggccctt tcgtcttcac tcgagtttac cactccctat cagtgataga
     5041 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     5101 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     5161 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     5221 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     5281 atcagtgata gagaaaagtg aaagtcgggc tcggttcccg ggtcgagatc cgtatcacga
     5341 ggccctttcg tcttcactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     5401 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     5461 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     5521 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     5581 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     5641 aaaagtgaaa gtcgggctcg gttcccgggt cgagatccgt atcacgaggc cctttcgtct
     5701 tcactcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     5761 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     5821 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     5881 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     5941 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     6001 gggctcggtt cccgggtcga gatccgtatc acgaggccct ttcgtcttca ctcgagttta
     6061 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     6121 agagaaaagt gaaagtcgag tttaccactc cctatcagtg mtagagmaaa gtgaaagtcg
     6181 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     6241 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     6301 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcggg ctcggttccc
     6361 gggtcgagat ccgtatcacg aggccctttc gtcttcactc gagtttacca ctccctatca
     6421 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     6481 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     6541 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     6601 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     6661 cactccctat cagtgataga gaaaagtgaa agtcgggctc ggttcccggg tcgagatccg
     6721 tatcacgagg ccctttcgtc ttcactcgag tttaccactc cctatcagtg atagagaaaa
     6781 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     6841 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     6901 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     6961 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     7021 tgatagagaa aagtgaaagt cgggctcggt tcccgggtcg agatccgtat cacgaggccc
     7081 tttcgtcttc actcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     7141 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     7201 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     7261 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     7321 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     7381 tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac gaggcccttt cgtcttcact
     7441 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     7501 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     7561 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     7621 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     7681 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgggct
     7741 cggttcccgg gtcgagatcc gtatcacgag gccctttcgt cttcactcga gtttaccact
     7801 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     7861 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     7921 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     7981 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     8041 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgggctcgg ttcccgggtc
     8101 gagatccgta tcacgaggcc ctttcgtctt cactcgagtt taccactccc tatcagtgat
     8161 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     8221 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     8281 agtgatagag aaaagtgaaa gtcgagttta ccmctcccta tcagtgatag agaaaagtga
     8341 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     8401 cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc ccgggtcgag atccgtatca
     8461 cgaggccctt tcgtcttcac tcgagtttac cactccctat cagtgataga gaaaagtgaa
     8521 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     8581 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     8641 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     8701 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     8761 gagaaaagtg aaagtcgggc tcggttcccg ggtcgagatc cgtatcacga ggccctttcg
     8821 tcttcactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     8881 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     8941 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     9001 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     9061 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     9121 gtcgggctcg gttcccgggt cgagatccac tagttctaga cccagctttc ttgtacaaag
     9181 tggttgatgg ggatccgaat tcgagctcga ggtcgacggt atcgataagc ttgatatcga
     9241 attcctgcag cccgggcagg gatccactag ttctagaatt cgagctcccg ggtactcgag
     9301 gtcgacatgc ccgccgtgac cgtcgagaac ccgctgacgc tgccccgcgt atccgcaccc
     9361 gccgacgccg tcgcacgtcc cgtgctcacc gtgaccaccg cgcccagcgg tttcgagggc
     9421 gagggcttcc cggtgcgccg cgcgttcgcc gggatcaact accgccacct cgacccgttc
     9481 atcatgatgg accagatggg tgaggtggag tacgcgcccg gggagcccaa gggcacgccc
     9541 tggcacccgc accgcggctt cgagaccgtg acctacatcg tcgacgcggc cagatggtaa
     9601 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     9661 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     9721 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9781 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9841 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9901 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9961 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10021 tgttcttcta gtgtagccgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    10081 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10141 ccacagaatc aggggataac gcaggaaaga acatggaagt tcctatactt tctagagaat
    10201 aggaacttcc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10261 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10321 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10381 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10441 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10501 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10561 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10621 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10681 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10741 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10801 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10861 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10921 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10981 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    11041 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    11101 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11161 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11221 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11281 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11341 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11401 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11461 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11521 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11581 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11641 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11701 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11761 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11821 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11881 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11941 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    12001 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    12061 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    12121 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    12181 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    12241 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    12301 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    12361 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    12421 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat
    12481 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    12541 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    12601 tcccagtcac gacgttgtaa aacgacggcc agtgaattcg agctcggtac agggttccgc
    12661 aagctctagt cgagccccag ctggttcttt ccgcctcaga agccatagag cccaccgcat
    12721 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
    12781 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
    12841 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
    12901 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgagctcta gagtcgactt
    12961 aattacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
    13021 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
    13081 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
    13141 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
    13201 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
    13261 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
    13321 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
    13381 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
    13441 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
    13501 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
    13561 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
    13621 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc
    13681 atggtggccg tacgtcacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga
    13741 ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc
    13801 aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca
    13861 taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg
    13921 tgcctggacg gcgcccggta ctccgtggag tcacatgaag cgacggctga ggacggaaag
    13981 gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat
    14041 tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc
    14101 gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc
    14161 agagcaggcc ggccagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg
    14221 ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca
    14281 aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa
    14341 aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg
    14401 agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc aggccaggcc
    14461 tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact ctcccaccca
    14521 cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga agtaattcaa
    14581 ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt
    14641 acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
    14701 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
    14761 cagtttaccc cgcgccacct tctctaggca ccggatcaat tgccgacccc tccccccaac
    14821 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gccaattccc
    14881 actcctttca agacctagaa ggtccattag ctgcaaagat tcctctctgt ttaaaacttt
    14941 atccatcttt gcagctagct tggctggacg taaactcctc ttcagacctg aagttcctat
    15001 actttctaga gaataggaac ttcggaattc gatatcaagc gtaccgggcc ccatcaa
//
