LOCUS       sequence-342556-       10494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of recombinant proteins in plants thanks to very 
            efficient translational efficiency. Empty vector, clone ORF using 
            Bsa1 sites. Download fully annotated map under Resource 
            Information..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Peyret H, Brown JKM, Lomonossoff GP
  TITLE     Improving plant transient expression through the rational design of 
            synthetic 5' and 3' untranslated regions.
  JOURNAL   Plant Methods. 2019 Sep 18;15:108. doi: 10.1186/s13007-019-0494-9. 
            eCollection 2019.
  PUBMED    31548848
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2019-09-18"; volume: "15:108. doi"; pages: " 
            10.1186/s13007-019-0494-9. eCollection 2019"
COMMENT     Sequence Label: pHREAC
FEATURES             Location/Qualifiers
     source          1..10494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     164..183
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     308..330
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     322..339
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     323..339
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        809..854
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1130..1382
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        1530..1875
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3348..3524
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4090..4269
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4122..4305
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4326..5120
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4380..4399)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4990..5009
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      5506..5758
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5849..5873
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      6241..6872
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(6962..8110)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8409..9203)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(9793..9810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10044..10063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttg tgaaccatca cccaaatcaa
       61 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
      121 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
      181 gagcgggcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      241 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      301 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgttaga gggagctctg
      361 atcactgagc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      421 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
      481 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cttaattaag
      541 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgagaag atagtggaaa
      601 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      661 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
      721 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
      781 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
      841 ttcatttgga gaggtttaag agacgcaacc acaacgctct aacgcaatca atctacatta
      901 tattaaacgt ctctaaaaag agacctaggt aactgactag ggtctctcgc tctggtttca
      961 ttaaattttc tttagtttga atttactgtt attcggtgtg catttctatg tttggtgagc
     1021 ggttttctgt gctcagagtg tgtttatttt atgtaattta atttctttgt gagctcctgt
     1081 ttagcaggtc gtcccttcag caaggacaca aaaagatttt aattttattg atcgttcaaa
     1141 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     1201 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     1261 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     1321 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     1381 tcggcgcgcc agcttggcgt aatcatggtc atagctgttg cgattaataa ttccacgcgg
     1441 atcgcattga gaacccggaa tatcgcgaaa caggtacccc cctactccaa aaatgtcaaa
     1501 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatttcggga
     1561 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
     1621 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatgcc
     1681 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     1741 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg
     1801 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     1861 catttggaga ggacagccca agcttcgact ctagaggatc cccttaaatc gatatgagca
     1921 ccgccaagat gagcgctggt gagttctcta agacctacgg caccaaggat agccggtctg
     1981 tgaatgattg ctacgccatc tacagcggca acggcatcaa cttcatcaac ctgttcatgc
     2041 acaccaacac cggcatcaag agcgccttct ctattaacga ccttggccgg aacgaggaca
     2101 ttaagcttca tgaggccgag atcatcaaca gcttgcaccc gtactcattc ttcgacaagt
     2161 tcggcctgga tatcatcctg tgcaaccacg tgatggacat catcgtgact aagcctggcc
     2221 ttaagaacac cggatgcaag ttccagatgc acaaccagat cttcaacccg aatgaggata
     2281 ttctggctaa gacccctggc atcatcagcg aagagaactt ctacgacctg agcaagatca
     2341 agcctaaggg catgacacct ttcgactggt acatcaacga gtgcaagaag tacgacttct
     2401 acatcagcga caacggcgac atctctctgg attacggttt ccctgtgatg ggcaagacca
     2461 cctcttattg gagagagaac atcccgcgag agaagatcat ctccgttaag cagaagtgcc
     2521 tgccgaacac ctctgctctt accaacagga ttctgagcct ttctaccgtg agggctatcc
     2581 agattgcttc tgagctggct tctgaaaaga ccgtggttct tgcttctagg cagaggctgg
     2641 atctggacat caagtctcag taccggatct cattccctgg tgttcaagat gagggcgctt
     2701 tcactaggac tttctgcatc cctatggaca agaccgctag gatcgtttgc ttctacgcta
     2761 agacctccgt ggacgtgtca aatgagagga ccaccttgac catcaagatc gtcaacaaga
     2821 ccgtcgagag caactacatg ggtcctgtgc ctaaggatca catgtactgc gataagagca
     2881 tcggcgctag gattggtctt gtggatattg tgaggggcga cccgaactac aatcagatga
     2941 ttgctcggga aatgatcagc gtccacacca actttgctct gaggctttct gaggctctga
     3001 agaagccggt catcgttttc aagatgtacg agaaagagct gaacttcgag acttacgacc
     3061 tgtccggtcg gtctttgtct taccagaagg acagcagcgg caatatctac ttcctgtcta
     3121 ggacccttga gatcctgccg aagtctcttt ctaccctgac ctacctgaag aacatctctc
     3181 ctgcctgctg gaaagaatcc atcagcatgc agcatttcta catcggggag caagaagagg
     3241 gaccttctgg ttctaatctg gccgagtctg aagagaaggt gcagactgct taggaattcg
     3301 gtacgctgaa atcaccagtc tctctctaca aatctatctc tctctatttt ctccataaat
     3361 aatgtgtgag tagtttcccg ataagggaaa ttagggttct tatagggttt cgctcatgtg
     3421 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     3481 atttctaatt cctaaaacca aaatccagta ctaaaatcca gatctcctaa agtccctata
     3541 gatctttgtc gtgaatataa accagacacc ggacgactaa acctggagcc cagacgccgt
     3601 tcgaagctag aagtaccgct taggcaggag gccgttaggg aaaagatgct aaggcagggt
     3661 tggttacgtt gactcccccg taggtttggt ttaaatatga tgaagtggac ggaaggaagg
     3721 aggaagacaa ggaaggataa ggttgcaggc cctgtgcaag gtaagaagat ggaaatttga
     3781 tagaggtacg ctactatact tatactatac gctaagggaa tgcttgtatt tataccctat
     3841 accccctaat aaccccttat caatttaaga aataatccgc ataagccccc gcttaaaaat
     3901 tggtatcaga gccatgaata ggtctatgac caaaactcaa gaggataaaa cctcaccaaa
     3961 atacgaaaga gttcttaact ctaaagataa aagatggcgc gtggccggcc atgagcggag
     4021 aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg
     4081 gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg gagtttaatg
     4141 agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat
     4201 cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt
     4261 atccaattag agtctcatat tcactctcaa tccaaataat ctgcaccgga tctggatcgt
     4321 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4381 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4441 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4501 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4561 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4621 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4681 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     4741 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4801 acgaagaaca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     4861 ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     4921 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     4981 aggacatagc gttggctacc cgtgatattg ctgaagaact tggcggcgaa tgggctgacc
     5041 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5101 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5161 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5221 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5281 cttcgcccac gggatctctg cgaaacaggc ggtcgaaggt gccgatatca ttacgacagc
     5341 aacggccgac aagcacaacg ccacgatcct gagcgacaat atgatcgcgg cgtccacatc
     5401 aacggcgtcg gcggcgactg cccaggcaag accgagatgc accgcgatat cttgctgcgt
     5461 tcggatattt tcgtggagtt cccgccacag acccggatga tccccgatcg ttcaaacatt
     5521 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     5581 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     5641 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     5701 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     5761 gggccggccc tcactggtga aaagaaaaac caccccagta cattaaaaac gtccgcaatg
     5821 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     5881 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     5941 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     6001 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     6061 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aaatatcatc
     6121 tccctcgcag agatccgaat tatcagcctt cttattcatt tctcgcttaa ccgtgacaga
     6181 gtagacaggc tgtctcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt
     6241 agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc
     6301 acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa
     6361 agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc
     6421 ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct
     6481 caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc
     6541 gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg
     6601 cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc
     6661 cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc
     6721 atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc
     6781 tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc
     6841 cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctcggtctt gccttgctcg
     6901 tcggtgatgt acactagtcg ctggctgctg aacccccagc cggaactgac cccacaaggc
     6961 cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc
     7021 ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat
     7081 ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc
     7141 tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga
     7201 atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc
     7261 aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt
     7321 ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc
     7381 attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct
     7441 cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct
     7501 gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca
     7561 cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt
     7621 tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg
     7681 gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt
     7741 tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt
     7801 ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg
     7861 ccgcctcctg ttcaagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag
     7921 ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc
     7981 cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg
     8041 catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg
     8101 tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca atgtgccctt
     8161 attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta tcggcaatga
     8221 agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga atcttgccct
     8281 gcacgaatac cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa
     8341 aacacttgat gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca
     8401 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
     8461 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
     8521 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
     8581 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
     8641 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
     8701 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
     8761 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
     8821 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
     8881 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
     8941 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
     9001 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
     9061 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
     9121 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
     9181 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
     9241 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     9301 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
     9361 tatcgacgga gccgattttg aaaccacaat tatgggtgat gctgccaact tactgattta
     9421 gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct
     9481 gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc
     9541 tctgctctca ctgccgtaaa acatggcaac tgcagttcac ttacaccgct tctcaacccg
     9601 gtacgcacca gaaaatcatt gatatggcca tgaatggcgt tggatgccgg gcaacagccc
     9661 gcattatggg cgttggcctc aacacgattt tacgtcactt aaaaaactca ggccgcagtc
     9721 ggtaactatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     9781 ctattccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    10201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcaggtaa
    10321 cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gggcccccgg
    10381 cgccagatct ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
    10441 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttag
//
