LOCUS       sequence-34256-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: pLenti-EF1a-Backbone(NG)
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..74)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(96..245)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(270..290)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctcctcgc ccttgctcac tgggccagga ttctcctcga cgtcaccgca tgttagcaga
       61 cttcctctgc cctctccact gcctctagag ttaatcagca tgtccaggtc gaaatcatca
      121 agggcgtcac tgccgagcat gtcgaggtca aagtcatcaa gggcatccga accaagcatg
      181 tcaaggtcaa aatcatcgag ggcgtcactt cccagcatat ccagatcaaa atcgtccaat
      241 gcgtcagccc gtccggaacc gctggcctcc acctttctct tcttcttggg gctagctgac
      301 gcgcgagttt ggtccccttc atgcatgggc gagggcgcgt ccaaatccct ctccagcgag
      361 tctgcgaacg catgaaggct cgcctggtca ggtgtctgag ttgacgtagg tgaaggcttc
      421 gcgcgtttca taccgctcgc ttggagaatc ctgtcccacc gctgggaggc gggaggcagt
      481 gtgcccgagc gggcctcgag ttctgtgaca ccgactctac gaaagagctg cagcagtccg
      541 tgtctcgaca taccaaattg agtcatggcg tcatcgaacg cttgtgcggg gtgggagtga
      601 cactggaaga atccgagcac gcggaccact tgcgcgtgat ctgccactcg atgtgatgtc
      661 ctctcgggaa tccttctgtt ggtccgctta atcaatgcag gcgcgtgcgg gagccccttc
      721 ttcaccgcat ccaaggcggg tcgtccgccg aggcaggcca gcgctacaag atgatcatta
      781 gtgagtgcgg ccagcgcggg gtccggcctc gaaagctggg ccacgattga ctccagtgcg
      841 ggtctgcccc cgccattgga tgcaatagcc actacctgct c
//
