LOCUS       sequence-34258-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: pLenti-EF1a-Backbone(HD)
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..55)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(77..226)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(251..271)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctgggccagg attctcctcg acgtcaccgc atgttagcag acttcctctg ccctctccac
       61 tgcctctaga gttaatcagc atgtccaggt cgaaatcatc aagggcgtca ctgccgagca
      121 tgtcgaggtc aaagtcatca agggcatccg aaccaagcat gtcaaggtca aaatcatcga
      181 gggcgtcact tcccagcata tccagatcaa aatcgtccaa tgcgtcagcc cgtccggaac
      241 cgctggcctc cacctttctc ttcttcttgg ggctagctga cgcgcgagtt tggtcccctt
      301 catgcatggg cgagggcgcg tccaaatccc tctccagcga gtctgcgaac gcatgaaggc
      361 tcgcctggtc aggtgtctga gttgacgtag gtgaaggctt cgcgcgtttc ataccgctcg
      421 cttggagaat cctgtcccac cgctgggagg cgggaggcag tgtgcccgag cgggcctcga
      481 gttctgtgac accgactcta cgaaagagct gcagcagtcc gtgtctcgac ataccaaatt
      541 gagtcatggc gtcatcgaac gcttgtgcgg ggtgggagtg acactggaag aatccgagca
      601 cgcggaccac ttgcgcgtga tctgccactc gatgtgatgt cctctcggga atccttctgt
      661 tggtccgctt aatcaatgca ggcgcgtgcg ggagcccctt cttcaccgca tccaaggcgg
      721 gtcgtccgcc gaggcaggcc agcgctacaa gatgatcatt agtgagtgcg gccagcgcgg
      781 ggtccggcct cgaaagctgg gccacgattg actccagtgc gggtctgccc ccgtcatggg
      841 atgcaatagc cactacctgc tc
//
