LOCUS       sequence-342581-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE006
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgcagt ctccggcaca atctagcgca ccgactgtgg aactcgattc tggtgccatc
       61 gcgggctcta ccgctgatgg cgttgtgtcc tttaaaggca tcccatacgc tgctccgccg
      121 gtgggtaatc tgcgttggcg cgctccacag ccggttgcga gctggactgg tgttcgtgct
      181 gcgactgaat atggctacga ctgcatccag ctgccgctgg aaggtgatgc ggctgcatct
      241 ggtggtgaaa tgtctgaaga ttgtctggtc ctgaacgtat ggcgcccggc tgaaattgca
      301 ccgggcgaac gcctgccagt tctggtttgg atccacgggg gtggctttct gaatggtagc
      361 gcagcggccc cgatctatga cggcaccgcg tttgcacagc agggtctggt agtagtaagc
      421 ttcaactacc gtctgggtcg tctgggtttc tttgcgcacc cggctctgac cgcggcgaac
      481 gagggtccgc tgggtaacta cgggctgatg gaccagatcg ctgcgctgga atgggtgcag
      541 cgtaatatcg ctgcattcgg tggtgaccca gcacgtatta ccctcatggg ccaatcggcg
      601 ggcggtatta gcgtaatgta ccacctgacc gcacctgaat ctcaaggtct gttccaccag
      661 gcggctgttc tgtccggtgg cggtcgtact tatctgctgg gtctgcgtaa cctgcgtgaa
      721 agcaccgatg cgctgccaag cgcagaacag tctggtctgg ctttcggtcg tcgtttcggc
      781 attcgtggcc gcggccgtgc tgctctgcgt tctctgagat ctctgagcgc cgaagaagtt
      841 aacggtgatc tgtcgatggc agctctggtt gagaaaccgg ccgattatgc aggtctcgag
//
