LOCUS       sequence-342585-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE008
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggttt tccttggctg gcagggctca ccggtgcagc tgccggcaca cgcgggcgaa
       61 caagcaccga gcccggttga gccactcaac ctgcctgatc cggcacgtcc gggagcttac
      121 ccggtagcgc tgttaactta cggctctggc caagataaac tgcgtcagga gtacgcgcaa
      181 ggtgcagctc tgctgacccc gagcgtggac gcgtctctgc tgctggaagg ctggtcctct
      241 ctccgtaccg cttattgggg tttttcccct gccgaactgc cgctgaatgg tcgtgtgtgg
      301 tatccgcagg cggaagggcg ttttccgctg gttatcgcag tacacggcaa ccaccctatg
      361 gaggaaacct cagagagtgg ctatgactac ctgggcgaac tgctggcttc ccgcggtttt
      421 atcttcgtgg ctgttgacga aaacttcctt aatatctccg catggggtga tgtgctgttt
      481 ttcaaccgcc tggaaggtga aagcgatgcg cgtggatggc tggttctgga acatcttcgt
      541 ctgtggcaaa gctggaacga acaaccaggc aacccgttct atcagcgcgt agatctgaac
      601 cagatcgcgc tgctgggcca ctcccgtggt ggagaagcaa ttgttatcgc cgcagcattc
      661 aaccgcctgt cccactaccc ggacaatgcg gccctgagtt tcgattacgg cttcaagatt
      721 cgcagcctga tcgcgctggc tccagccgac ggccagtacc agccaggcgg tctgcctacc
      781 ccgctgcagg atgttaacta cctgctgctg catggttccc acgacatgga cgtgctgact
      841 atgatgggcg cggcgccgtt cgaacgtctg accttcagcg gccaggatga cttcttcaag
      901 tctgcagttt acatctacgg cgctaaccac ggtcagttca actctgtgtg gggcaataag
      961 gatatcgcag aaccgatccc acgtctgtac aatctgcgtc agctgctgcc gcagaccgaa
     1021 cagcaacgta tcgcgcaggt actgattagc gcattcctgg aagatactct gcgtggtgaa
     1081 cgtgcctatc gtccgctgtt tcaactcgag
//
