LOCUS       sequence-342587-         423 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE009
FEATURES             Location/Qualifiers
     source          1..423
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgctgg ttcgcatcgg tgaacaggaa gacgcggttg ctgcgctgga attcctgctg
       61 cagcgcgacg aaatcgacac cgagcgtatt gctctggctg gttactcttt cggtgcgttc
      121 gttggtctgg cagcactgaa tggcaacgaa aacattaaag cactggtggg tgtttcaccg
      181 cctctgactc tgttcgaatt cagctacctc aaaaattgca cgaaaccaaa actgctgatc
      241 atcggtgaca tggaccaatt cactccactg aaggttttca aagaattcta cgagaagatc
      301 ccggaaccga aaaacaaacg catcattgaa ggtgcggatc acttctattg gggttacgag
      361 aacgaagttg gtcaggtagt tgctgatttt ctgaaaaaaa ccttcaaaaa catcccgctc
      421 gag
//
