LOCUS       sequence-342589-        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE010
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggttg atatcactgg caacggtatg gctgctaccg cgccgaccga cgaacgtatt
       61 gtagacaaac ctctgcctca gccgcagatt cgttctggta acgttcgtgc aatgccggcg
      121 gctcgcaagc tggcgcagga gcacggtatt gacctgtcca ctctgaccgg tagcggtcca
      181 ggtggtgtta tcgttaaaga ggacgtcgaa cgtgcaatca ccgctcgtgc tgttcctgta
      241 tctccgctgc agcgtgtcaa cttttattct gccggttatc gtctggacgg tctgctgtac
      301 accccgcgtc acctgccagc tggtgaacgt agaccgggtg tcgttctcct ggtgggttac
      361 acttacctca aaactatggt aatgccggac atcgcgaaag ttctgaacgc tgcgggttac
      421 gttgccctgg ttttcgacta ccgcggcttc ggcgaatccg agggcccgcg cggtcgtcta
      481 atcccgttag aacaagtagc tgatgcacgt gcagcgctga cctttctggc ggaacagtca
      541 atggttgatc cggatcgtct cgcggtaatt ggcatttctc tgggtggtgc acatgcaatt
      601 accactgctg cactggatca gcgtgtccgc gcggtcgtgg ctctggaacc gccaggccat
      661 ggtgcgcgtt ggctgcgtag cctgcgtcgt cactgggaat ggcgtcagtt cctgtctcgt
      721 ctggctgaag atcgtcgtca gcgcgtgcta agcggtggca gcaccatggt tgacccgctg
      781 gagatcgttc tgccagaccc ggagtctcag gctttcctgg accaagttgc cgcagaattt
      841 ccgcagatga aagtgacgct gccgctggaa tctgccgaag cactgatcga atatgtgtcc
      901 gaagacctcg ccggccgtat cgctccgcgt ccactgctga tcattcactc tgacgccgac
      961 cagctggttc cggttgcgga agctcaggcg atcgcagagc gcgcgggctc ttctgcacag
     1021 ctggagatca ttccaggcat gtcccatttc aattgggtaa tgccaggcag cccgggcttc
     1081 actcgtgtta ctgattctat cgttaaattc ctgcgtaaca ccctgccggt atctgcggac
     1141 aatctcgag
//
