LOCUS       sequence-342591-         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE011
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggttc cgctgatcct gaacgtgcat ggtggtccgg caggcgtttt ccagcagacc
       61 tttaccggtg gtcgcagcat ctatccgatc gctaccttcg cagcgcgcgg ttacgctgtt
      121 ctgcgtccga acccgcgcgg ttcctctggt tatggtgtgg aattccgccg tgccaacctc
      181 aaagattggg gtggcatgga ctaccaggac ctcatggctg gtgttgacaa agtaatcgaa
      241 atgggtgtgg cggactcctc ccgtctgggt gttatgggtt ggtcctatgg tggttttatg
      301 acgtcttgga tcgtaactca gacaaaccgt ttcaaagcgg cttcagcagg cgctccggtc
      361 accaacctga cgtcattcac tactaccgcc gacatcccgg cgttcatccc ggactatttc
      421 ggcggccagt tctgggactc tccggaagtg taccgtgcac actccccaat ttcgttcgtg
      481 aaatctgtca ccactcctac tatgatccag cacggtaccg cagacatgcg tgttccgatc
      541 agccagggtt tcgaattcta caacgctctc aaagctcgtg gcatcccgac ccgcatgctc
      601 gag
//
