LOCUS       sequence-342593-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE012
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggtgc caagcgcggg tgtaggtctt tctggcgtcc tccatctgcc ggctggcgtt
       61 tcccgcccgg tgctgttcct gcatggtttc acgggcaaca agacggaaag cggtcgtttg
      121 tacaccgaca tggcgcgcgt tctgtgttct gcgggctacg cagccttgcg tttcgatttt
      181 cgtggtcacg gtgatagccc tctgccattc gaggaatttc gtatcagtct ggcagttgaa
      241 gacgcccgta acgcggccgg tttcctgaaa aacgtaccgg aagtggacgg aactcgcttt
      301 ggtgtagtgg gcttgtctat gggtggcggc gtggcagtga gcctggcggc tggtcgcgaa
      361 gacgttggtg cgctcgtgct gctttctccg gctctggatt ggcctgaact cttccagcgt
      421 gcgcgtggct tctttcgtgc ggaagagggc tacgtgtact ggggcccgca ccgtatgcgc
      481 gatgtttacg ctatggaaac catgaacttc tctgtaatgg gcctggccga agaaatccaa
      541 gcgccgactc tgatcatcca ctctgttgat gacatggttg ttccgattag tcaagccaaa
      601 cgcttctatg aaaaactgaa agtagaaaaa aagtttatcg agatcgaaca cggtggtcac
      661 gtttttgatg actacaacgt gcgtcgccgt atcgagcagg aggttctcga ctgggtgaaa
      721 cgccacctgc tcgag
//
