LOCUS       sequence-342597-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE014
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggttc catccgcggg tgtaggcctg tctggcgttc ttcacctgcc ggcaggcgta
       61 agccgcccgg tgctgtttct gcacggtttc accggtaaca aaaccgaatc cggccgcctt
      121 tatactgaca tggctcgtgt tctgtgttct gccgggtatg cagcgctgcg ctttgacttt
      181 cgttgccatg gggattcccc gctgccattc gaggaattcc gcatctcact ggcggttgaa
      241 gatgcgcgta atgccgctgg ctttctgaaa aatgttcctg aagttgatgg caccaaattc
      301 ggcgtggttg gtctgtctat gggaggtggt gttgctgttt cgctcgccgc gggccgtgag
      361 gatgtaggtg ctctggtact gctgtctccg gcccttgatt ggccggagct gttccagcgc
      421 gcacgtggct tcttccgcgc ggaagaaggt tacgtgtact ggggtccgaa ccgtatgcgt
      481 gatgtatacg caatggagac catgaacttc agcgtgatgg gcctggcaga agaaattaaa
      541 gcgccgactc tgatcattca ctcggtggat gatgtggtag tgccgatcag tcaggctaaa
      601 cgtttctacg aaaaactgaa agttgaaaaa aaatttatcg aaatcgaaca gggcggccac
      661 gtgtttgaag attacaacgt tcgtcgtcgt atcgaacgtg aagttctgga ctgggtgaag
      721 cgccatttac tcgag
//
