LOCUS       sequence-342599-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE015
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgggct ttaccggcaa caaagccgag gctggtcgtc tgtacaccga catggctcgt
       61 gtcctgtgtg cggctggcta cgctgcgctg cgcttcgact tccgttgtca tggcgactcc
      121 ccgctgccgt ttgaagaatt ccgcatttcg tacgcggtag aagacgcacg taacgcggct
      181 tctttcctga agattcaacc gtccgtagac ggttctcgtt tcgccgtaat cggcctgtcg
      241 atgggtggtg gtgtagccgt gagtctggca gcgggtcgtg atgatgtagc tgccctcgtg
      301 ctcctgtctc cggctctgga ttggccggaa ctggcggcgc gcattccgca gccgaaggtg
      361 gaaggcggct acgtgtacat gggtccaaac cgcatgaagg tggagtgtgt tacggaaacc
      421 atgaaattca ctgtcatgga cctggcagaa cgtgttaaag ctcccactct gatcattcat
      481 gccgctgatg atatggttgt tccgattagc caatccaaac gcttctatga aaaactgaaa
      541 gtagagaaaa agtttatgga gatcgaacgt agcggtcatg ttttcgatga ttacaacgta
      601 cgccgccgtg tcgaagcgga agttcttgat tggatcaaaa aacacctgct cgag
//
