LOCUS       sequence-342601-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE016
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggacg gctgcatcga ggatctgcgc ttcatcgagt tcgatggttt ccgtttagca
       61 tctaccatcc accgtccggc cattgcgacc tcttccgctg tgctgatgct gcatggcttt
      121 acgggtaacc gcatcgaggt caaccgtctg tacgtagaca tcgcacgtcg cctgtgttcc
      181 gaaggtatgg ttgttctgag actggactac cgcggccacg gcgaatcttc cttgccgttc
      241 gaagaattca aaatcggtta cgccctggag gatggtggta aagccctgga agttctgcag
      301 aaactgttta acccggtgcg tatcggcgtc gtcggtttct ctctgggtgg ttatgtagct
      361 attcacctgg caagccgcta tcgtggtgcg atctcttctc tggcgctgct ggccccgggt
      421 atcaagatgg atgagctggc aactgaactt gcccgcaaac tgtccctgga aggcgacttc
      481 tacatcgtac gtgcactgaa aattcgccgc gaaggtatcg aaagcatgat ccgtagcccg
      541 tccgccatga tttatgcaga caccgttgat atcccggtgc tgatcatcca cgcgaaaaac
      601 gacagcgcgg tgccttacat ccacagcatc gaattttacg aaaaaatccg cagccagaaa
      661 aaacgcatcg ttatcctgga cgaaggtggt cacactttcg aactgcacca catccgtgat
      721 cgcgtgatcg aagaagtggt tgcgtggttc cgcgaaaccc tgctgtacac gctcgag
//
