LOCUS       sequence-342603-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE017
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggttg acatcaccgg caacggcatg gcggccacgg cccccaccga cgaacgcatt
       61 gttgataaac cgctgcctca gccgcaaatt cgctctggta atgtgcgtgc aatgccggct
      121 gcacgtaaac tggcacagga acatggcatc gacctgtcta ccctgactgg ctctggcccg
      181 ggtggcgtga tcgtaaaaga agatgttgaa cgtgcgatca ccgctcgtgc cgttccggtt
      241 tctccgctgc agcgtgtgaa cttctattct gcaggctacc gtctcgatgg tctgctgtat
      301 actccgcgtc acctgccagc gggcgaacgt cgcccgggtg tcgtcctgct ggtgggttac
      361 acgtacctta aaaccatggt gatgccagat atcgccaaag tattaaatgc ggcgggctat
      421 gttgcactgg tttttgacta ccgtggcttt ggtgaatctg aagggccgcg tggccgcctg
      481 atcccgctgg aacaagtggc agacgcgcgt gcggcgttga ctttccttgc ggaacagtcc
      541 atggtagatc cggaccgtct ggctgtgatc ggcatcagcc tgggtggtgc gcacgcgatc
      601 actaccgccg ctctggatca gcgcgtgcgc gcagtcgtcg cacttgaacc gcctggtcac
      661 ggcgctcgct ggctgcgctc gctgcgtcgt cactgggaat ggcgccagtt cctctctcgt
      721 ttagcagagg atcgtcgtca gcgcgttctg tctggcggct ctaccatggt tgatcctctg
      781 gaaattgtcc tgccggatcc agagtcccag gccttcctgg atcaggttgc cgcagaattc
      841 ccgcagatga aagtgaccct gccgctggag tccgcggaag cgctgatcga atatgttagc
      901 gaagacctgg ctggacgtat tgctccgcgc ccgctgctga tcatccacgg ggacgcagat
      961 cagctcgttc cggttgctga ggcccaagct attgccgaac gtgcaggcag cagtgcgcag
     1021 ctggaaatca tcccaggtct cgag
//
