LOCUS       sequence-342605-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE018
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgctga ttcgcccggt tgctttccgt aacatgaacc aacagatcat cggcatcctg
       61 cacacgccgg acaacattaa acctggcgaa aaaaccccgg gtatcctgat gctgcacggc
      121 ttcaccggta acaaaaccga agctcatcgt ctctttgttc atgtagcccg ttctctgagc
      181 gaatatggtt tcatcgtact tcgtttcgac ttccgtggtt ccggcgattc tgatggtgaa
      241 ttcgaagata tgacccttcc tggcgaagtc tccgacgcgg aacgtgctct gaccttcctg
      301 ctccgtcgcc gtaacatcga ccgcgatcgc gtgggcgtca tcggtctgtc catgggtggc
      361 cgtgttgccg ccatcctggc atctaaagat aagcgtgtta aatttgcggt cctgtactcc
      421 ccggcactgg gtccgttgcg tgatcgttcc ctgtcgttca tgagccgtga gaaaatcgaa
      481 cgcctgaaca gcggtgaggc ggtagaattt ttcgctgaag gctggtacat caaaaaaact
      541 ttcttcgaaa ccgtggacta catcgttcca ctcgacatca tggactcgat ccgtgttccg
      601 gtgctcatcg tccatggtga tcgtgatccg attattccgg tggaagaagc aattcgagcg
      661 tacgaaaaaa tcaaaggtgt aaacaagaag aacgaactgt atattgttcg tggtggtgac
      721 cataccttct ccaaaaaaga acacacccag gaagttatca aaaaaaccct ggactggatc
      781 cgtgccctct ccgtctccga aggctccatc gttctgtttc gcctgctgga actcgag
//
