LOCUS       sequence-342607-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE019
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgctga ttcgtccggt taccttccgc aatatgaacc agcagattat tggcatcctt
       61 cacactccgg acaacatccg tctgaatgaa aaagtaccgg gtatcctgat gttccatggc
      121 ttcactggta ataaaactga agcgcaccgc ctgtttgtgc acgtggctcg ttctctgtcc
      181 gaacatggtt tcatcgtgct gcgtttcgac ttccgcggaa gcggtgatag cgatggtgaa
      241 ttcgaagaca tgaccctgcc gggtgaagtt agcgacgcag agcgcgcgct gacctttctg
      301 ttgcgccagc gtaacgttga taaaaaccgt attggtgtaa tcggtctgtc catgggtggc
      361 cgtgttgcgg cgattctggc aagcaaggac cggcgcgtta aattcgctgt cctgtacagc
      421 ccggcgctgg gtccgctgcg cgatcgttct ctgtctttca tgagcaaaga aaaaattgaa
      481 cgtctgaact ccggtgaggc agtggaattc ttcgctgaag gttggtatat caaaaaagca
      541 ttctttgaga ccgtggacta tattgtcccg ctggacatca tggattccat taaagttccg
      601 gttttgatcg ttcatggcga caaagacccg ctcattccgg ttggtgaggc tatccgtgca
      661 tacgaaaaaa tcaaaggtgt taacgagaaa aatgagctgt acattgtacg tggcggtgat
      721 cacaccttct ccaaaaaaga acacaccctg gaggtaatca agaaaacttt ggactggatc
      781 cgtagcctgg gcattctcga g
//
