LOCUS       sequence-342609-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE020
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgctga ctatcacagc gatcatctac ctcctggcca ccattatcgc agccatcctc
       61 cttgttgtat atattatttc ctcctccgcg tctaaaaaac tggccacgcc gccgcgaaaa
      121 accggttcct ggtcaccgcg cgatctgggt ttcgagtacg agaaagtgga agtgaaaacc
      181 tccgatggac tgactctgcg tggctggctg attccgcgcg gctccgaaaa aaccgtcatc
      241 gtgatccacg gttacacttc ttgcaagtgg gatgaatggt atatgaagcc ggttatcaac
      301 atcctggcac gtcacgattt caacgtcgtt gcatttgata tgcgtgcgca cggcgaatct
      361 gatggcgaaa agactaccct gggctaccgt gaggtagatg acatcggcgc aattattaat
      421 tacctgaaag aacgtggcct ggcaagtcgc ctgggcatca ttggctactc gatgggcggt
      481 gctatcactc tgatgtccct ggcgcgctac gaggaactga aagcgggtgt ggccgactcg
      541 ccgtacatcg acattcgtgc gtcgggtaaa cgttggatta atcgcgttgg cgctcctctg
      601 cgctacatcc tcctggcatc gtacccgctg atcatgcgtc ttactgcctc ccgtactggc
      661 gcatctccgg aaaaactggt tatgtaccaa tatgccaagt cgatcaccaa accactgctt
      721 atcattggtg gtcagcagga cgacctggtg gccatcgacg aagtacgtaa attctatgaa
      781 gaagttaaaa aagtgaactc caatgtcgaa ctgtgggaaa ccacctccaa acacgtttct
      841 gccatccaag attacccgcg tgaatacgaa gagcgtatcg tcggcttctt caaccgctgg
      901 ctgctcgag
//
