LOCUS       sequence-34261-e         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: monomer-NI
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(600..617)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(600..616)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tttacacgta cttagtcgct gaactgaccc cagagcaggt cgtggcaatc gcctccaaca
       61 ttggcgggaa acaggcactc gagactgtcc agcgcctgct tcccgtgctg tgccaagcgc
      121 acggacgtca atcgagttcg tacctaaggg cgacacaaaa tttattctaa atgcataata
      181 aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac atgtataatt
      241 ttgatatcaa aaactgattt tccctttatt attttcgaga tttattttct taattctctt
      301 taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa tagtttaatt
      361 ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg cttttttctc
      421 atttataagg ttaaataatt ctcatatatc aagcaaagtg acaggcgccc ttaaatattc
      481 tgacaaatgc tctttcccta aactcccccc ataaaaaaac ccgccgaagc gggtttttac
      541 gttatttgcg gattaacgat tactcgttat cagaaccgcc cagggggccc gagcttaaga
      601 ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac gcaaaaggcc atccgtca
//
