LOCUS       sequence-342613-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE022
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcgc gcgcagcgcc gatttcgccg ctgcagcgtg taaacttcta ctctgcaggt
       61 tatcgcttgg atggcctgct gtatactcct cgtcatctgc cggcgggtga acgtcgtccg
      121 ggcgttgtgc tgctggtcgg ttacacctac ttaaaaacca tggtgatgcc ggatatcgct
      181 aaagtgctga acgctgccgg ttacgtagct ctggtcttcg attaccgtgg ctttggtgaa
      241 agcgaaggtc cacgtggtcg tttgatcccg ctggagcagg tagctgacgc gcgtgccgca
      301 ctgaccttct tggctgaaca gagcatggtc gatccggacc gtctggcagt cattggcatc
      361 agcctgggcg gcgcacacgc aatcaccaca gcggcgctgg accaacgcgt acgtgcagtc
      421 gttgcgattg aaccaccggg tcacggcgcg cactggctgc gttcccttcg tcgtcactgg
      481 gagtggtccc agttcctgtc tcgcttgacc gaagatcgtc gtcagcgcgt tctgtccggt
      541 gtcagcagca ctgttgaccc actggaaatc gttctgccag acccagaatc tcaggccttt
      601 ctggaccagg tggcggcgga atttccgcag atgaaagtga cgcttccact ggaatcggct
      661 gaggcgctga ttgaatacgt cccggaagac ctggcaggtc gtatcgcccc gcgcccgctg
      721 ctgctcgag
//
