LOCUS       sequence-342615-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE023
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcca cggttctggt aattccgaaa ctcggtctga cgatgactga aggccgtgtc
       61 ggccgttggc tgaaacagtt aggcgagccg gtacaggctg gcgaaccagt gcttgaagta
      121 gagaccgaaa aactgaccgt agaggtcgag gccccagcta gcggcatcct ggcatatatt
      181 ctggcggaag aaggcgttgt tctgccggtg actgctccgg tggcagtgat cgccgagccg
      241 ggtgaagctg ttgacctggc ttctctcctg ccggctactt ccggtgcggc agcaaccccg
      301 gttatggctg catccagcac catgcaagaa caggctcgtg cacagggtcc aaccccgacc
      361 ggcgaaatcc gcgcaacccc ggctgctcgt aagctggcac gtgaccacgg cattgacctg
      421 gcacgtgttc gcggcaccgg tcctggtggc cgtattacgg cggaggatgt agaacgttac
      481 ctggccagcc agggtaccgc ttggcctcgc ggtgaaccgg ttcgtttctg gtcagacggt
      541 ctggctctgg ctggtgaact gttcttacct ccgagtaccg atacggcggt tcctggtgtt
      601 gtgctatgca ccggcattca gggcctgaaa gagctcggta tgccgctgct ggcgcaggca
      661 ctggcagatg ccgggtacgc tgctctgatt tttgattatc gcggcttcgg tgcttccgag
      721 ggtccgcgtg gccgtctgct gccacaggaa cgcattcgtg atgcacgtgc agcgctcact
      781 ttcctggaaa ctcacccact tatcgatcgt acccgcctgg ccatcctggg cctgtcgctc
      841 ggtggcgcac acgcgctttc cctggcggca atcgacgatc gcgttcaggc ttgtatcgca
      901 atcgccccgc tgaccaacgg acgccgctgg ctgcgttctc tgcgtgctga atggcagtgg
      961 cgtgttctcg ag
//
