LOCUS       sequence-342619-         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE025
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggtac gtcgtccgaa caacactacc tttactgcac agctgtacta tccggctacg
       61 gcgactggtg acaacgctcc gtacgacggc tctggcgcac cgtatccggc ggtgtcgttt
      121 ggtcacggtt ttcttcaacc gcccgaacgc tatcgctcta tcctggaaca ccttgctagc
      181 tggggttacc tgaccatcgc caccgaatct ggtcaagagc tgtttccgaa ccaccgtgcg
      241 tacgctgaag atatgcgtta ctgcctgacc tacctggaag aacagaatgc cgatccggcg
      301 tcttggttat ttggccaggt ggccactgct caattcggta tcagcggtca ctccatgggt
      361 gggggtgcct ccatcctggc agctgccgct gatgcacgta ttaaagcggt tgcaaacctg
      421 gcagccgcag aaactaaccc gagcgcgatc caagcgagcc cgaacatcac cgttcctcat
      481 tctctcatct ccggttccgc cgacactatc actccgttgt catccaatgg tctgcgtatg
      541 tacactgcgg gtctgcgtgc tgaagcagct gcacgccact ctcgtcgtct gggcctgcgc
      601 gttccgaaaa ctccgagcat ttttggctgc gatagcggca gcctgccacc gcgtcacgcg
      661 ctcgag
//
