LOCUS       sequence-342621-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE026
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgatct ggtatccggc tgttcgtgtc cgtggccaac cgcagcgtac cacgtaccag
       61 tacggcccac tgattgggga aggacgtgca tatcgcgacg cgccggcaga tctgcgcggc
      121 gccccatacc cgttactgat cttctctcac ggtctgggtg gtgcgcgtat ccagtccgtg
      181 ttctacgcag agcacctggc aagccatggt ttcgtcgtta tggcggccga tcacacgggt
      241 tcgacttttg ctgacctgct gcgtggccgt gcggatagca tcctggaatc gttcgcccgc
      301 cgcccgctgg aaatcctgcg tcagattgaa tacgcggcag cgttaaacgc agacgacgat
      361 acgctgcgtg gcgcgatcga tgcagagacc gtcggcgtaa caggccactc ttttggcggt
      421 tacactgccc tcgcggccgc aggtgctcaa ctgaatatca atgcgatccg tgaaggttgt
      481 gaaagcggca aactgccgga acagcagtgt ctgtttgtac gctccgaaga aatcatctgg
      541 cgtgcacgtg gcctgtctgc cgcccctgaa ggcctgtatc cgccgacgac tgacccgcgc
      601 atcaaagctg ttgtggcact ggcgccgtcg tctgcaccga ccttcggtga agcaggtgcc
      661 gctgctcttc gtgtaccgct gatgatcatt gtaggttcta aagaccaagc caccccgccg
      721 gagcgcgatt cctatccgat ctaccagagc gtgtcctctg ctcagaaagc gctggtagtc
      781 ttcgaaaacg ctggtcatta tatcttcgtg gaacagtgcg tcccggcttt gattgcactg
      841 ggtcgtttcg aacagtgctc tgacctagta tgggacatgc agcgtgctca cgatctgatt
      901 aatcacttcg cgactgcctt ctttctgcac gccctgaaag gtgatccggc ggccaaagcg
      961 gcgctggacc cgactgcggt tcagttcatc ggcatcactt accgccgtga cggcgcttgg
     1021 ctcgag
//
