LOCUS       sequence-342623-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE027
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgattg tgctgctgct taacttcgac gtggaatata aacgtatcaa attcaacggt
       61 gactatattg acatctacaa acctaaagcc gaaggtaact acccgttcgt tatcttttct
      121 ggtggcatga actccccatc ctcgcgttac gaatctttcg gtaagttcct ggctagtaac
      181 ggtttcatca ccattatccc ggattacaaa ggttggctct tcctgctgct gattccgctg
      241 aagatcctgc gcatcatcga taacttaaat aaaatcgact ctagcattaa gaacgaaggc
      301 tgcctcgggg gccacagtct gggcgcgtac ttcagtatga tcgtctccta taagcgttct
      361 agcgtgaaat gcctgttcct cttctctccg ccggcgttat tcctgaacta ctctaaaatt
      421 aaagtgccgg ttctcatctt cgccggtacc aacgatgaaa tcacgaaatt tgaagcgaac
      481 cagaaaatta tctatgaaca cctgaaaact cagaaaaaac tggttctgat tgagggtggt
      541 aatcacaacg gttatatgga tcgttgggat ttcgtcgagg cgctgactga tggttacctc
      601 ggtatcgagc acaaaaagca gctggaaatt gttcgcgatt ccgttctgaa gttcctgaaa
      661 gaaatcttac tgaaactcga g
//
