LOCUS       sequence-342625-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE028
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgcaag taattcagca gaccgttaca ctgcaaaaaa cccaactgcg cctgaccaag
       61 gaaggcttcg ttaccaatta tcgtttcccg gtggatttct actaccctga ttctccggaa
      121 tctttcccgg taattctgat ctctcatggt tttggctcgg tccgcgaaaa cttccgcact
      181 ctggcacagc atctggcctc tcacggcttc ctggtagccg ttccgcagca catcggctcg
      241 gatctgcagt accgtcaaga gctgatcaaa ggtactttat cctccgcact gtccccagtt
      301 gaatttttgg cgcgtccgac cgacctgtct accatcattg actatctgca ggcgactcag
      361 aacaccggct cctggcagaa gcgtgcaaat ctgcagcaga tcggcgttat cggtgatagt
      421 ctgggcggta ccactgctct gacgattggt ggtgcaccgc tggatattcc gcgtctgcag
      481 actaaatgta cctcggacaa cgttattgtg aacgttgccc tgatcctgca atgccaggcc
      541 tcgttcctgc cgccgagcga atacaacctg gctgattccc gtgtcaaagc cgttattgcc
      601 acgcacccgc tgatctcagg cattttttct ccggactctc tggcgaaaat tcagatccca
      661 gtgatgatta ccgcgggcaa ctttgacatc atcaccccgc tcgag
//
