LOCUS       sequence-342627-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE029
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgaagg tgaagtctaa acccctgact ctgtacaacg tttctggcga ccgtattact
       61 gcggatgtcc actttgttga atcctttctg ccggcgccgg ttgtgatcta cagccacggc
      121 tttcttggtt tcaaagactg gggcttcatc ccgtacgtgg cagaacgttt cgcggaaaac
      181 ggcttcgtct tcgttcgttt taacttttcc cacaacggca tcggtgagaa cccgaacaaa
      241 attaccgaat ttgataaact ggcgaaaaac accattagca agcagatcga agatctgact
      301 gcagttatcg aatacgtatt ctccgatgaa tttggtgttc tcaacgatgg tcaactgttc
      361 ctcctgggcc actctggcgg cggtggcatc tccatcatca aggcagtcga agacgaacgt
      421 gttcgtgctc tggctctgtg ggcaagcatc tccactttcc gtcggtactc taagcatcag
      481 attgaagaac tggaaaaaaa cggctatatc ttcgttcgtg tcccggactc tgttattcag
      541 gtcaaaattg aaaagattgt ttacgatgat ttcgtcgaga actctgaacg ctacgatatc
      601 atcaaagcca tttctaagct gaaaatccca atcctgatcg tgcacggcac tgcggacgct
      661 attgtcccgc tggcagaggc ggaaaaactg cgtaattcca acccggaata taccaaactg
      721 gtcctgatct ccggtgccaa tcacctgttc aacgtaaaac atccgatgga gcacagcact
      781 gaccaactgg ataaagcgat cgacgagacc gttctgttct tcaagaagat tatcgaaaac
      841 aaaaaggcgg atctcgag
//
