LOCUS       sequence-342629-         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE030
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgcaaa ccgtgaccag catgctgaaa gacctggacg cggtaattac tcaggtttca
       61 gaaaaatttc cgcagattga caacaagcgc gtctgtctga tcggtcactc tcagggtgcg
      121 tacgtatcct tcctgcatgc gaccaaagat gaacgtatta aatgcctggt ctcctggatg
      181 ggtcgtctgt cggacctgaa agaattttgg tctaagctgt ggttcgacga gatcgaacgc
      241 aaaggctata tctacgagtg ggattacaaa atcaccaaga aatatgtgcg tgatagcctg
      301 aaatacaatc tgtcaaaagc tgcatggcgt atcaaagtgc cgaccctgct gatttatggt
      361 gaactggacg atatcgtgcc accttctgaa ggtatgaaat tctaccgcaa catcaaatct
      421 ccgaaaaaaa tcgttattgt aaaggatctg aaccacacct tctctggtga aaaagccaaa
      481 aaatccgtta tccgcatcac tctgaaatgg ctgtctaaat ggctcaagcg cctggacctc
      541 gag
//
