LOCUS       sequence-342631-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE031
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgctga aaatcatcga agattttgcg agtctggaca ccggtgttaa agtgttctat
       61 cgttgtatcc tgccggaatc tttcaaagaa ctggcgatcg tgtctcatgg tttcacctcg
      121 cactctggtt tctacatcca tattggcaag gaattggcgt cctatggcta tggtgtttgc
      181 atccacgatc agcgcggtca cggtcgtacc gcgcagaacc tggaacgtgg ctacgtagat
      241 tcttttaacg atttcctggt ggacctggaa actttcacta tgcatgttca gcgcgttttc
      301 ggtggtgaac gcaccgtcct aatcggacac agcatgggcg gtctgattgt tctgctgtat
      361 gcgggcaaat acggtcgcgt tggcgacgcc gtagttgcag tggctccggc cgttctgatt
      421 ccggaaaccc gccgttttag cactctgatc tttgccacca ttgcctccat cctgtttccg
      481 cgtaagcgta ttgaactccc gtttaccgaa cagcagattg aggaaggtat gaaacgtatg
      541 gaccgtgagc tcctggaagc aatgggtaaa gatgaactgg tactgcgtga caccaccatt
      601 aaactgctgg ttgaaatctg gaaagcgtcg cgtgaatttt ggcgctacgt ggaacgtatc
      661 cagatcccga ccctgctgat ccacggcgaa aaagacaata tcatcccgat tgaagctagc
      721 cgccgtacct attctcgcct gaaaactctg aaaaaagagc tgatcgttta cccggaatgc
      781 ggccattctc cgctgcatga aatcggttgg cgtgaacgta tcaaaaacat ggtagaatgg
      841 atccgcaaca acatcctcga g
//
